Literature summary for 3.2.1.28 extracted from

Carroll, J.D.; Pastuszak, I.; Edavana, V.K.; Pan, Y.T.; Elbein, A.D.
A novel trehalase from Mycobacterium smegmatis - purification, properties, requirements (2007), FEBS J., 274, 1701-1714.

Search for more literature for 3.2.1.28

Activating Compound

Activating Compound	Comment	Organism	Structure
alpha,alpha-trehalose	the enzymatic activity increases with increasing concentrations of alpha,alpha-trehalose in the incubations up to about 50 mM	Mycolicibacterium smegmatis
arsenate	-	Mycolicibacterium smegmatis
phosphate	-	Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21	Mycolicibacterium smegmatis

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	50% inhibition at 20 mM	Mycolicibacterium smegmatis
ATP	90% inhibition at 20 mM	Mycolicibacterium smegmatis
castanospermine	50% inhibition at 0.5 mg/ml	Mycolicibacterium smegmatis
diphosphate	more than 50% inhibition at 8 mM and complete inhibition at 16 mM	Mycolicibacterium smegmatis
additional information	40 mM sodium fluoride and trehazolin have no inhibitory effect	Mycolicibacterium smegmatis
Polyphosphate	-	Mycolicibacterium smegmatis
sodium orthovanadate	80% inhibition at 10 mM	Mycolicibacterium smegmatis
UTP	90% inhibition at 10 mM	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
20	-	alpha,alpha-trehalose	at 37°C	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Mycolicibacterium smegmatis	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolutely required for activity, optimum activity occurring at concentrations of 3.5-4 mM	Mycolicibacterium smegmatis
additional information	Mg2+ can not be replaced by Ca2+, Mn2+ or Zn2+	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71000	-	SDS-PAGE	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-
Mycolicibacterium smegmatis b11	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate fractionation, phenyl-Sepharose DE-52 column chromatography and Sephacryl S-300 gel filtration	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.227	-	cell extract, at 37°C	Mycolicibacterium smegmatis
36.99	-	after 162fold purification, at 37°C	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha,alpha-trehalose + H2O	highly specific for alpha,alpha-trehalose	Mycolicibacterium smegmatis	2 D-glucose	-	?
alpha,alpha-trehalose + H2O	highly specific for alpha,alpha-trehalose	Mycolicibacterium smegmatis b11	2 D-glucose	-	?
additional information	does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside	Mycolicibacterium smegmatis	?	-	?
additional information	does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside	Mycolicibacterium smegmatis b11	?	-	?

Subunits

Subunits	Comment	Organism
multimer	20 or more subunits, gel filtration	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
MSMEG 4528	-	Mycolicibacterium smegmatis
trehalase	-	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	-	Mycolicibacterium smegmatis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.5	8	the trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)