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Literature summary for 3.2.1.28 extracted from
- Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization (2005), Plant Physiol. Biochem., 43, 355-361.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibits the acid trehalase by 70% | Phaseolus vulgaris |  |
| Fe3+ | inhibits the acid trehalase by 50% | Phaseolus vulgaris | |
| Hg+ | inhibits the acid trehalase by 95% | Phaseolus vulgaris | |
| validamycin A | inhibits the acid trehalase | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.109 | - |
alpha,alpha-trehalose | pH 6.3, 37°C | Phaseolus vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates the acid trehalase | Phaseolus vulgaris | |
| K+ | activates the acid trehalase | Phaseolus vulgaris | |
| Li+ | activates the acid trehalase | Phaseolus vulgaris | |
| Mg2+ | activates the acid trehalase by about 30% | Phaseolus vulgaris | |
| Mn2+ | activates the acid trehalase by about 30% | Phaseolus vulgaris | |
| Na+ | activates the acid trehalase by about 30% | Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
| 45000 | - |
x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | Phaseolus vulgaris | - |
2 D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acid trehalase 425fold from root nodules, by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and native PAGE | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root nodule | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 175.7 | - |
purified acid trehalase | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
| alpha,alpha-trehalose + H2O | preferred substrate of acid trehalase | Phaseolus vulgaris | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Phaseolus vulgaris | 2 D-glucose | - |
? | |
| melibiose + H2O | - |
Phaseolus vulgaris | D-glucose + D-galactose | - |
? | |
| additional information | no activity of acid trehalase with lactose and turanose | Phaseolus vulgaris | ? | - |
? | |
| raffinose + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| sucrose + H2O | - |
Phaseolus vulgaris | D-glucose + D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000 + x * 45000, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid trehalase | - |
Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Phaseolus vulgaris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | - |
Phaseolus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, stable up to | Phaseolus vulgaris |
| 45 | 50 | loss of 40% activity, purified acid trehalase | Phaseolus vulgaris |
| 60 | - |
nearly complete inactivation of purified acid trehalase | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | - |
acid trehalase | Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | 6.6 | - |
Phaseolus vulgaris |
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