Literature summary for 3.2.1.28 extracted from

de Aquino, A.C.M.; Peixoto-Nogueira, S.C.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.de L.
Characterization of an acid trehalase produced by the thermotolerant fungus Rhizopus microsporus var. rhizopodiformis: biochemical properties and immunochemical localisation (2005), FEMS Microbiol. Lett., 251, 169-175.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	alpha,alpha-trehalose	pH 4.5, 45°C	Rhizopus microsporus var. rhizopodiformis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	tightly packed	Rhizopus microsporus var. rhizopodiformis	9986	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51500	-	2 * 60000, SDS-PAGE, 2 * 51500, deglycosylated enzyme, SDS-PAGE	Rhizopus microsporus var. rhizopodiformis
60000	-	2 * 60000, SDS-PAGE, 2 * 51500, deglycosylated enzyme, SDS-PAGE	Rhizopus microsporus var. rhizopodiformis
105000	-	gel filtration	Rhizopus microsporus var. rhizopodiformis

Organism

Organism	UniProt	Comment	Textmining
Rhizopus microsporus var. rhizopodiformis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	carbohydrate content is 72%	Rhizopus microsporus var. rhizopodiformis

Purification (Commentary)

Purification (Comment)	Organism
native enzyme to homogeneity	Rhizopus microsporus var. rhizopodiformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha,alpha-trehalose + H2O	-	Rhizopus microsporus var. rhizopodiformis	2 D-glucose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, SDS-PAGE, 2 * 51500, deglycosylated enzyme, SDS-PAGE	Rhizopus microsporus var. rhizopodiformis

Synonyms

Synonyms	Comment	Organism
acid trehalase	-	Rhizopus microsporus var. rhizopodiformis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Rhizopus microsporus var. rhizopodiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	1 h, stable, purified enzyme	Rhizopus microsporus var. rhizopodiformis
60	-	half-life of purified enzyme is 1.3 min	Rhizopus microsporus var. rhizopodiformis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	-	Rhizopus microsporus var. rhizopodiformis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rhizopus microsporus var. rhizopodiformis	-	-	6.7

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Release 2023.1 (January 2023)