Literature summary for 3.2.1.28 extracted from

Martins, A.S.; Peixoto, D.; Paiva, L.M.C.; Panek, A.D.; Paiva, C.L.A.
A simple method for obtaining reusable reactors containing immobilized trehalase: characterization of a crude trehalase preparation immobilized on chitin particles (2006), Enzyme Microb. Technol., 38, 486-492.

No PubMed abstract available

Search for more literature for 3.2.1.28

Application

Application	Comment	Organism
biotechnology	immobilization of the crude enzyme on chitin resulting in stable, specific, and reusable reactors for application in other biotechnological processes, the immobilized enzyme shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the enzyme in strain Mph2 from plasmid pTRE11	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	alpha,alpha-trehalose	pH 5.5, 30°C, immobilized enzyme	Escherichia coli
1.32	-	alpha,alpha-trehalose	pH 5.5, 30°C, soluble enzyme	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Escherichia coli	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	x * 58000, recombinant enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli Mph2	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
preparation of periplasmic fraction of recombinant Escherichia coli	Escherichia coli

Storage Stability

Storage Stability	Organism
immobilized crude recombinant enzyme on chitin shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days	Escherichia coli

Subunits

Subunits	Comment	Organism
?	x * 58000, recombinant enzyme	Escherichia coli

Synonyms

Synonyms	Comment	Organism
trehalase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	immobilized enzyme	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	immobilized enzyme	Escherichia coli

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Release 2023.1 (January 2023)