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Literature summary for 3.2.1.26 extracted from

  • de Almeida, M.; Guimaraes, V.; Falkoski, D.; de Camargo, B.; Fontes-Santana, G.; Maitan-Alfenas, G.; de Rezende, S.
    Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus (2018), J. Food Biochem., 42, e12551 .
No PubMed abstract available

Application

Application Comment Organism
food industry invertase 2 has potential to be applied in food industry since its product, inverted sugar, is used in candies and syrup production, while fructooligosacharides are prebiotics, low calorie and noncariogenic sweeteners Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2 mM, 50.3% loss of activity Aspergillus terreus
Ca2+ 2 mM, 36.7% loss of activity Aspergillus terreus
fructose competitive inhibitor for invertase 2 Aspergillus terreus
Mg2+ 2 mM, 31.1% loss of activity Aspergillus terreus
SDS 2 mM, 89.5% loss of activity Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2
-
sucrose 55°C, pH 4.6, invertase 2 Aspergillus terreus
9.8
-
sucrose 55°C, pH 4.6, invertase 1 Aspergillus terreus
16.4
-
raffinose 55°C, pH 4.6, invertase 2 Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus CCT 4083
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information intracellular invertase 2 and invertase 1 do not hydrolyze inulin Aspergillus terreus ?
-
?
additional information intracellular invertase 2 and invertase 1 do not hydrolyze inulin Aspergillus terreus CCT 4083 ?
-
?
raffinose + H2O invertase 2 has higher affinity for sucrose than for raffinose Aspergillus terreus beta-D-fructose + melibiose
-
?
raffinose + H2O invertase 2 has higher affinity for sucrose than for raffinose Aspergillus terreus CCT 4083 beta-D-fructose + melibiose
-
?
sucrose + H2O invertase 2 also shows transfructosylating activity producing fructooligosaccharides. Invertase 1 does not show transfructosylating activity Aspergillus terreus beta-D-fructose + alpha-D-glucose
-
?
sucrose + H2O invertase 2 also shows transfructosylating activity producing fructooligosaccharides. Invertase 1 does not show transfructosylating activity Aspergillus terreus CCT 4083 beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, invertase 2, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
invertase 1
-
Aspergillus terreus
invertase 2
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
invertase 2 Aspergillus terreus
60
-
invertase 1 Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life: 44 h, invertase 2 Aspergillus terreus
55
-
half-life: 14.9 h, invertase 2 Aspergillus terreus
60
-
half-life: 2 h, invertase 2 Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
invertase 1 Aspergillus terreus
4.6
-
invertase 2 Aspergillus terreus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
61.5
-
fructose 55°C, pH 4.6, invertase 2 Aspergillus terreus