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Literature summary for 3.2.1.26 extracted from

  • Tauzin, A.S.; Sulzenbacher, G.; Lafond, M.; Desseaux, V.; Reca, I.B.; Perrier, J.; Bellincampi, D.; Fourquet, P.; Leveque, C.; Giardina, T.
    Functional characterization of a vacuolar invertase from Solanum lycopersicum: post-translational regulation by N-glycosylation and a proteinaceous inhibitor (2014), Biochimie, 101, 39-49.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
N119D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
N184D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
N516D the mutation dramatically affects the folding of the protein. The mutant is inactive Solanum lycopersicum
N52D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
N52D/N119D/N184D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
N52D/N119D/N184D/N516D the mutations dramatically affect the folding of the protein. The mutant is inactive Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
SolyVIF non-competitive inhibition Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14
-
sucrose at pH 4.5 and 50°C Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
Solanum lycopersicum 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60566
-
x * 60566, calculated from amino acid sequence Solanum lycopersicum
69114
-
x * 69114, mass spectrometry Solanum lycopersicum
70000
-
x * 70000, deglycosylated protein, SDS-PAGE Solanum lycopersicum
79000
-
x * 79000, glycoprotein, SDS-PAGE Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum P29000 cultivar Red Moneymaker
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has four N-glycosylation sites. Glycosylation of N516 is crucial for recombinant enzyme stability Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephacryl S-200 gel filtration Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
flower highest expression Solanum lycopersicum
-
fruit high expression in mature fruit Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
Solanum lycopersicum fructose + glucose
-
?

Subunits

Subunits Comment Organism
? x * 60566, calculated from amino acid sequence Solanum lycopersicum
? x * 69114, mass spectrometry Solanum lycopersicum
? x * 70000, deglycosylated protein, SDS-PAGE Solanum lycopersicum
? x * 79000, glycoprotein, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
TIV-1
-
Solanum lycopersicum
Vacuolar invertase
-
Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1384
-
sucrose at pH 4.5 and 50°C Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
99
-
sucrose at pH 4.5 and 50°C Solanum lycopersicum