Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
N119D | the mutant shows reduced activity compared to the wild type enzyme | Solanum lycopersicum |
N184D | the mutant shows reduced activity compared to the wild type enzyme | Solanum lycopersicum |
N516D | the mutation dramatically affects the folding of the protein. The mutant is inactive | Solanum lycopersicum |
N52D | the mutant shows reduced activity compared to the wild type enzyme | Solanum lycopersicum |
N52D/N119D/N184D | the mutant shows reduced activity compared to the wild type enzyme | Solanum lycopersicum |
N52D/N119D/N184D/N516D | the mutations dramatically affect the folding of the protein. The mutant is inactive | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SolyVIF | non-competitive inhibition | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
sucrose | at pH 4.5 and 50°C | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Solanum lycopersicum | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60566 | - |
x * 60566, calculated from amino acid sequence | Solanum lycopersicum |
69114 | - |
x * 69114, mass spectrometry | Solanum lycopersicum |
70000 | - |
x * 70000, deglycosylated protein, SDS-PAGE | Solanum lycopersicum |
79000 | - |
x * 79000, glycoprotein, SDS-PAGE | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | P29000 | cultivar Red Moneymaker | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has four N-glycosylation sites. Glycosylation of N516 is crucial for recombinant enzyme stability | Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sephacryl S-200 gel filtration | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | highest expression | Solanum lycopersicum | - |
fruit | high expression in mature fruit | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
stem | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Solanum lycopersicum | fructose + glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60566, calculated from amino acid sequence | Solanum lycopersicum |
? | x * 69114, mass spectrometry | Solanum lycopersicum |
? | x * 70000, deglycosylated protein, SDS-PAGE | Solanum lycopersicum |
? | x * 79000, glycoprotein, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
TIV-1 | - |
Solanum lycopersicum |
Vacuolar invertase | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1384 | - |
sucrose | at pH 4.5 and 50°C | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Solanum lycopersicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
99 | - |
sucrose | at pH 4.5 and 50°C | Solanum lycopersicum |