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Literature summary for 3.2.1.26 extracted from

  • Lafraya, A.; Sanz-Aparicio, J.; Polaina, J.; Marin-Navarro, J.
    Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase (2011), Appl. Environ. Microbiol., 77, 6148-6157.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.26

Protein Variants

Protein Variants Comment Organism
D22N activity bleow detection limit with sucrose. Increase in transfructosylating activity Saccharomyces cerevisiae
F82W 71% of wild-type activity Saccharomyces cerevisiae
N21S 104% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
N228A 138% of wild-type activity Saccharomyces cerevisiae
N228A/N21S 20% of wild-type activity Saccharomyces cerevisiae
N228R 23% of wild-type activity, no transfructosylating activity Saccharomyces cerevisiae
N228R/N21S 4% of wild-type activity Saccharomyces cerevisiae
N24S 17% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
P205V 112% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
P205V/W19Y/N21S 3% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
W19Y 175% of wild-type activity with sucrose. Increase in transfructosylating activity Saccharomyces cerevisiae
W19Y/N21S 41% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
W19Y/N21S/N24S 2% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
W19Y/N24S 8% of wild-type activity with sucrose. Large increase in transfructosylating activity Saccharomyces cerevisiae
W287Y 4% of wild-type activity with sucrose. Increase in transfructosylating activity Saccharomyces cerevisiae
W291Y 32% of wild-type activity with sucrose. Increase in transfructosylating activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19
-
 sucrose mutant N24S, pH 4.8, 50°C Saccharomyces cerevisiae
38
-
 sucrose wild-type, pH 4.8, 50°C Saccharomyces cerevisiae
1300
-
 sucrose mutant N21S, pH 4.8, 50°C Saccharomyces cerevisiae
5000
-
 sucrose mutant W19Y/N21S, pH 4.8, 50°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Saccharomyces cerevisiae beta-D-fructose + alpha-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Suc2
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
360
-
 sucrose mutant N24S, pH 4.8, 50°C Saccharomyces cerevisiae
3300
-
 sucrose mutant W19Y/N21S, pH 4.8, 50°C Saccharomyces cerevisiae
3430
-
 sucrose wild-type, pH 4.8, 50°C Saccharomyces cerevisiae
4000
-
 sucrose mutant N21S, pH 4.8, 50°C Saccharomyces cerevisiae