Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 134% of initial activity | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, 64% residual activity | Thermotoga neapolitana | |
Cu2+ | 2 mM, 5% residual activity | Thermotoga neapolitana | |
Fe3+ | 2 mM, 0.8% residual activity | Thermotoga neapolitana | |
guanidinium hydrochloride | 2 M, 2% residual activity | Thermotoga neapolitana | |
Hg2+ | 2 mM, no residual activity | Thermotoga neapolitana | |
K+ | 2 mM, 79% residual activity | Thermotoga neapolitana | |
Urea | 2 M, 29% residual activity | Thermotoga neapolitana | |
Zn2+ | 2 mM, 0.8% residual activity | Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
sucrose | 85°C, pH 6.0 | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44298 | - |
1 * 47000, SDS-PAGE, 1 * 44298, MALDI TOF-MS | Thermotoga neapolitana |
47000 | - |
gel filtration and PAGE | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
DSM 4359 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51800 | - |
85°C, pH 6.0 | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Thermotoga neapolitana | D-fructose + ? | - |
? | |
raffinose + H2O | - |
Thermotoga neapolitana | D-fructose + ? | - |
? | |
sucrose + H2O | - |
Thermotoga neapolitana | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE, 1 * 44298, MALDI TOF-MS | Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 85 | retains at least 50% of initial activity for 6 h | Thermotoga neapolitana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7300000 | - |
sucrose | 85°C, pH 6.0 | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermotoga neapolitana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
520000 | - |
sucrose | 85°C, pH 6.0 | Thermotoga neapolitana |