Literature summary for 3.2.1.26 extracted from

Altenbach, D.; Rudino-Pinera, E.; Olvera, C.; Boller, T.; Wiemken, A.; Ritsema, T.
An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase (2009), Plant Mol. Biol., 69, 47-56.

Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment)	Organism
Pichia pastoris with vector pPICZalphaC	Allium cepa

Protein Variants

Protein Variants	Comment	Organism
F348L	wild-type activity	Allium cepa
L201M	wild-type activity	Allium cepa
N251C	wild-type activity	Allium cepa
S457N	wild-type activity	Allium cepa
V276Y	no activity	Allium cepa
W343Y	wild-type activity	Allium cepa
W440Y	almost doubled transglycosylation activity	Allium cepa
Y394F	no activity	Allium cepa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.8	-	sucrose	pH 5.6, 28°C, wild-type	Allium cepa
8.8	-	sucrose	pH 5.6, 28°C, W440Y	Allium cepa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	-	Allium cepa	5773	-

Organism

Organism	UniProt	Comment	Textmining
Allium cepa	O81083	-	-

Purification (Commentary)

Purification (Comment)	Organism
centrifugation of cell culture medium, supernatants diluted in 50 mM MES-buffer, pH 5.6	Allium cepa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	50 mM MES-buffer, pH 5.6, 20 min, 28°C	Allium cepa	D-fructose + D-glucose	at sucrose concentrations higher than 100 mM distinct but small amount of 1-kestose is found as transglycosylation product	?

Synonyms

Synonyms	Comment	Organism
acINV	Allium cepa invertase	Allium cepa
Vacuolar invertase	-	Allium cepa

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Release 2023.1 (January 2023)