Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-sorbitol cinnamic ester | intense hydrophobic interactions between cinnamoyl groups of the compound with related groups of the enzyme immobilizes the enzyme and enhances substrate affinity and hydrolysis rate, immobilization at pH 5.5, 4°C, 12 h | Zymomonas mobilis |
Application | Comment | Organism |
---|---|---|
food industry | production of invert sugar | Zymomonas mobilis |
Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21 (DE3) with invbpetK plasmid, 37°C, 2TY medium | Zymomonas mobilis |
General Stability | Organism |
---|---|
operational stability of immobilized invertase: 40% of the initial activity remaining after 45 cycles of 1 min duration, producing 90.6 mg sucrose within 45 min by 2.5 mg immobilized enzyme | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78 | - |
sucrose | immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 | Zymomonas mobilis | |
98 | - |
sucrose | free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 | Zymomonas mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
lysed cells centrifuged, supernatant purified by nickel-affinity chromatography | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O | sucrose | Zymomonas mobilis | D-glucose + D-fructose | - |
? | |
sucrose + H2O | - |
Zymomonas mobilis | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 51000, SDS-PAGE | Zymomonas mobilis |
Synonyms | Comment | Organism |
---|---|---|
re-INVB | free and immobilized enzyme | Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
immobilized and free enzyme | Zymomonas mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | immobilized enzyme: about 50% of maximal activity at 20°C, about 40% of maximal activity at 60°C, free enzyme: about 65% of maximal activity at 20°C, about 40% of maximal activity at 50°C, almost no activity at 60°C | Zymomonas mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | free enzyme: 50% activity after 20 min at 30°C and 10 min at 40°C | Zymomonas mobilis |
25 | 60 | immobilized enzyme: 50% activity after 3600 min at 4°C, after 150 min at 25°C, after 100 min at 30°C, after 70 min at 40°C | Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12000 | - |
sucrose | free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 | Zymomonas mobilis | |
50000 | - |
sucrose | immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
immobilized enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer | Zymomonas mobilis |
5.5 | - |
free enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer | Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | free enzyme: maximum activity at pH 5.5, about 70% activity at pH 5, about 40% activity at pH 6, activities below 20% at pH 4.5 and 7, no activity at pH 3-4 and 7.5 | Zymomonas mobilis |
3.5 | 7.5 | immobilized enzyme: maximum activity at pH 5, low activity at pH 5.5, about 50% activity at pH 4.5, no activity at pH 3-4 and 6-7.5 | Zymomonas mobilis |