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Literature summary for 3.2.1.26 extracted from

  • Chaouachi, M.; El Malki, R.; Berard, A.; Romaniuk, M.; Laval, V.; Brunel, D.; Bertheau, Y.
    Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum) (2008), J. Agric. Food Chem., 56, 1818-1828.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-sorbitol cinnamic ester intense hydrophobic interactions between cinnamoyl groups of the compound with related groups of the enzyme immobilizes the enzyme and enhances substrate affinity and hydrolysis rate, immobilization at pH 5.5, 4°C, 12 h Zymomonas mobilis

Application

Application Comment Organism
food industry production of invert sugar Zymomonas mobilis

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21 (DE3) with invbpetK plasmid, 37°C, 2TY medium Zymomonas mobilis

General Stability

General Stability Organism
operational stability of immobilized invertase: 40% of the initial activity remaining after 45 cycles of 1 min duration, producing 90.6 mg sucrose within 45 min by 2.5 mg immobilized enzyme Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
78
-
sucrose immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 Zymomonas mobilis
98
-
sucrose free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 Zymomonas mobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
SDS-PAGE Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
lysed cells centrifuged, supernatant purified by nickel-affinity chromatography Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O sucrose Zymomonas mobilis D-glucose + D-fructose
-
?
sucrose + H2O
-
Zymomonas mobilis beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
? 1 * 51000, SDS-PAGE Zymomonas mobilis

Synonyms

Synonyms Comment Organism
re-INVB free and immobilized enzyme Zymomonas mobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
immobilized and free enzyme Zymomonas mobilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 immobilized enzyme: about 50% of maximal activity at 20°C, about 40% of maximal activity at 60°C, free enzyme: about 65% of maximal activity at 20°C, about 40% of maximal activity at 50°C, almost no activity at 60°C Zymomonas mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 free enzyme: 50% activity after 20 min at 30°C and 10 min at 40°C Zymomonas mobilis
25 60 immobilized enzyme: 50% activity after 3600 min at 4°C, after 150 min at 25°C, after 100 min at 30°C, after 70 min at 40°C Zymomonas mobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12000
-
sucrose free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 Zymomonas mobilis
50000
-
sucrose immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 Zymomonas mobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
immobilized enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer Zymomonas mobilis
5.5
-
free enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer Zymomonas mobilis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7.5 free enzyme: maximum activity at pH 5.5, about 70% activity at pH 5, about 40% activity at pH 6, activities below 20% at pH 4.5 and 7, no activity at pH 3-4 and 7.5 Zymomonas mobilis
3.5 7.5 immobilized enzyme: maximum activity at pH 5, low activity at pH 5.5, about 50% activity at pH 4.5, no activity at pH 3-4 and 6-7.5 Zymomonas mobilis