Literature summary for 3.2.1.26 extracted from

Reca, I.B.; Brutus, A.; DAvino, R.; Villard, C.; Bellincampi, D.; Giardina, T.
Molecular cloning, expression and characterization of a novel apoplastic invertase inhibitor from tomato (Solanum lycopersicum) and its use to purify a vacuolar invertase (2008), Biochimie, 90, 1611-1623.

Inhibitors

Inhibitors	Comment	Organism	Structure
invertase inhibitor of Solanum lycopersicum	6.7 nM tomato invertase with 1-2.5 nM inhibitor retains more than 90% invertase activity, 3-4 nM less than 90% activity, 5 nM about 75%, about 6.7 nM about 60% activity, pH 4.9, 37 degrees Celsius, 100 mM sucrose as substrate, proteinaceous functional inhibitor is expressed in leaves, flowers, and green fruit, proteolytically cleaving the invertase	Solanum lycopersicum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	-	Solanum lycopersicum	5773	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	native PAGE, SDS-PAGE produced only fragments of 46.3, 27.7, 18.6, and 16.8 kDa, in red fruits additionally a 44 kDa fragment, and a 35 kDa fragment shown to be produced by in planta proteolysis of invertase	Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	P29000	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Solanum lycopersicum

Purification (Commentary)

Purification (Comment)	Organism
green and red tomato fruits homogenized separately in 10 mM NaOAc cold buffer, pH 4.6 containing 1 M NaCl, centrifuged, supernatant precipitated, resuspended in buffer, dialyzed, loaded onto invertase inhibitor of Solanum lycopersicum affinity column, washing with buffer, eluted with 50 mM potassium phosphate buffer, pH 7.5, fractions with invertase activity pooled, loaded onto Hiload 16/60 Superdex 75 column, eluted with 20 mM Na acetate, pH 4.6, containing 150 mM NaCl	Solanum lycopersicum

Purification (Comment)

Organism

green and red tomato fruits homogenized separately in 10 mM NaOAc cold buffer, pH 4.6 containing 1 M NaCl, centrifuged, supernatant precipitated, resuspended in buffer, dialyzed, loaded onto invertase inhibitor of Solanum lycopersicum affinity column, washing with buffer, eluted with 50 mM potassium phosphate buffer, pH 7.5, fractions with invertase activity pooled, loaded onto Hiload 16/60 Superdex 75 column, eluted with 20 mM Na acetate, pH 4.6, containing 150 mM NaCl

Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O	sucrose, 0.2 M sodium acetate buffer, pH 4.9, 37°C	Solanum lycopersicum	D-glucose + D-fructose	-	?

Subunits

Subunits	Comment	Organism
monomer	glycosylated monomer composed of several splitting fragments that have to be tightly associated for enzyme activity to occur	Solanum lycopersicum

Synonyms

Synonyms	Comment	Organism
TIV-1	-	Solanum lycopersicum
Vacuolar invertase	-	Solanum lycopersicum