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Literature summary for 3.2.1.26 extracted from

  • Alvaro-Benito, M.; de Abreu, M.; Fernandez-Arrojo, L.; Plou, F.J.; Jimenez-Barbero, J.; Ballesteros, A.; Polaina, J.; Fernandez-Lobato, M.
    Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose (2007), J. Biotechnol., 132, 75-81.
    View publication on PubMed
Search for more literature for 3.2.1.26

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 raffinose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
1.3
-
 1-kestose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
1.6
-
 nystose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
4.9
-
 sucrose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82500
-
 2 * 82500, gel filtration Schwanniomyces occidentalis
85000
-
 2 * 85000, SDS-PAGE Schwanniomyces occidentalis
165000
-
 gel filtration Schwanniomyces occidentalis

Organism

Organism UniProt Comment Textmining
Schwanniomyces occidentalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel chromatography Schwanniomyces occidentalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Schwanniomyces occidentalis ?
-
 ?
additional information no activity with maltose, lactose, turanose, leucrose, and palatinose Schwanniomyces occidentalis ?
-
 ?
nystose + H2O
-
 Schwanniomyces occidentalis ?
-
 ?
raffinose + H2O
-
 Schwanniomyces occidentalis D-fructose + melibiose
-
 ?
sucrose + H2O
-
 Schwanniomyces occidentalis D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 85000, SDS-PAGE Schwanniomyces occidentalis
homodimer 2 * 82500, gel filtration Schwanniomyces occidentalis

Synonyms

Synonyms Comment Organism
beta-fructofuranosidase shows also transfructosylating activity Schwanniomyces occidentalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
 Schwanniomyces occidentalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.9
-
 nystose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
10.9
-
 raffinose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
26.3
-
 1-kestose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis
80.5
-
 sucrose in 0.2M sodium acetate buffer (pH 5.6), at 50°C Schwanniomyces occidentalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Schwanniomyces occidentalis