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Literature summary for 3.2.1.26 extracted from
- Purification and some properties of beta-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404 (2007), J. Biosci. Bioeng., 103, 491-493.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by metal ions | Aureobasidium pullulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 230 | - |
sucrose | in 50 mM sodium acetate buffer (pH 5.0), at 30°C | Aureobasidium pullulans |  |
| 700 | - |
1-kestose | in 50 mM sodium acetate buffer (pH 5.0), at 30°C | Aureobasidium pullulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 430000 | - |
TSK Super SW3000 column HPLC | Aureobasidium pullulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium pullulans | - |
strain DSM 2404 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose DE52 column chromatography, Toyopearl butyl-650M column chromatography, and Sepharose CL-4B column chromatography | Aureobasidium pullulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.4 | - |
crude extract, at 30°C | Aureobasidium pullulans |
| 2180 | - |
after 51.4fold purification, at 30°C | Aureobasidium pullulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| sucrose + H2O | - |
Aureobasidium pullulans | D-glucose + D-fructose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-fructofuranosidase I | - |
Aureobasidium pullulans |
| FFase I | - |
Aureobasidium pullulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aureobasidium pullulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable at 45°C for 1 h | Aureobasidium pullulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aureobasidium pullulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable at pH 6-10 at 30°C for 1 h | Aureobasidium pullulans |
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