Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose | stimulates activity 2.68fold at 40 mM | Aspergillus ochraceus | |
EDTA | slightly stimulating at 1 mM | Aspergillus ochraceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 73% residual activity at 1 mM | Aspergillus ochraceus | |
Cu2+ | 53% residual activity at 10 mM; 94% residual activity at 1 mM | Aspergillus ochraceus | |
Hg+ | complete inhibition at 10 mM | Aspergillus ochraceus | |
Mg2+ | 79% residual activity at 10 mM | Aspergillus ochraceus | |
Na+ | 73% residual activity at 10 mM | Aspergillus ochraceus | |
NH4Cl | 25% residual activity at 10 mM | Aspergillus ochraceus | |
Zn2+ | 19% residual activity at 10 mM | Aspergillus ochraceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.66 | - |
inulin | in sodium acetate buffer, 100 mM, pH 4.5, at 60°C | Aspergillus ochraceus | |
7.37 | - |
raffinose | in sodium acetate buffer, 100 mM, pH 4.5, at 60°C | Aspergillus ochraceus | |
13.4 | - |
sucrose | in sodium acetate buffer, 100 mM, pH 4.5, at 60°C | Aspergillus ochraceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 20% stimulation of activity at 1 mM | Aspergillus ochraceus | |
Mg2+ | 50% stimulation of activity at 1 mM | Aspergillus ochraceus | |
Mn2+ | 57% stimulation of activity at 1 mM | Aspergillus ochraceus | |
Na+ | 35% stimulation of activity at 1 mM | Aspergillus ochraceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67500 | - |
2 * 67500, gel filtration | Aspergillus ochraceus |
79000 | - |
2 * 79000, SDS-PAGE | Aspergillus ochraceus |
135000 | - |
gel filtration | Aspergillus ochraceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ochraceus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 41% carbohydrate content | Aspergillus ochraceus |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose chromatography and Sephacryl S-200 gel filtration | Aspergillus ochraceus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
crude extract, at pH 4.5, 60°C | Aspergillus ochraceus |
42.5 | - |
after 7.1fold purification, at pH 4.5, 60°C | Aspergillus ochraceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Aspergillus ochraceus | D-fructose + ? | - |
? | |
raffinose + H2O | - |
Aspergillus ochraceus | fructose + melibiose | - |
? | |
sucrose + H2O | - |
Aspergillus ochraceus | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 79000, SDS-PAGE | Aspergillus ochraceus |
homodimer | 2 * 67500, gel filtration | Aspergillus ochraceus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-fructofuranosidase | - |
Aspergillus ochraceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus ochraceus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life is 60 min at 60°C | Aspergillus ochraceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus ochraceus |