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Literature summary for 3.2.1.26 extracted from

  • Guimaraes, L.H.; Terenzi, H.F.; Polizeli, M.d.; Jorge, J.A.
    Production and characterization of a thermostable extracellular beta-D-fructofuranosidase produced by Aspergillus ochraceus with agroindustrial residues as carbon sources (2007), Enzyme Microb. Technol., 42, 52-57.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
D-glucose stimulates activity 2.68fold at 40 mM Aspergillus ochraceus
EDTA slightly stimulating at 1 mM Aspergillus ochraceus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 73% residual activity at 1 mM Aspergillus ochraceus
Cu2+ 53% residual activity at 10 mM; 94% residual activity at 1 mM Aspergillus ochraceus
Hg+ complete inhibition at 10 mM Aspergillus ochraceus
Mg2+ 79% residual activity at 10 mM Aspergillus ochraceus
Na+ 73% residual activity at 10 mM Aspergillus ochraceus
NH4Cl 25% residual activity at 10 mM Aspergillus ochraceus
Zn2+ 19% residual activity at 10 mM Aspergillus ochraceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.66
-
inulin in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus
7.37
-
raffinose in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus
13.4
-
sucrose in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 20% stimulation of activity at 1 mM Aspergillus ochraceus
Mg2+ 50% stimulation of activity at 1 mM Aspergillus ochraceus
Mn2+ 57% stimulation of activity at 1 mM Aspergillus ochraceus
Na+ 35% stimulation of activity at 1 mM Aspergillus ochraceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67500
-
2 * 67500, gel filtration Aspergillus ochraceus
79000
-
2 * 79000, SDS-PAGE Aspergillus ochraceus
135000
-
gel filtration Aspergillus ochraceus

Organism

Organism UniProt Comment Textmining
Aspergillus ochraceus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 41% carbohydrate content Aspergillus ochraceus

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose chromatography and Sephacryl S-200 gel filtration Aspergillus ochraceus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
crude extract, at pH 4.5, 60°C Aspergillus ochraceus
42.5
-
after 7.1fold purification, at pH 4.5, 60°C Aspergillus ochraceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
Aspergillus ochraceus D-fructose + ?
-
?
raffinose + H2O
-
Aspergillus ochraceus fructose + melibiose
-
?
sucrose + H2O
-
Aspergillus ochraceus D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 79000, SDS-PAGE Aspergillus ochraceus
homodimer 2 * 67500, gel filtration Aspergillus ochraceus

Synonyms

Synonyms Comment Organism
beta-D-fructofuranosidase
-
Aspergillus ochraceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus ochraceus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life is 60 min at 60°C Aspergillus ochraceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus ochraceus