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Literature summary for 3.2.1.26 extracted from

  • Janer, C.; Rohr, L.M.; Pelaez, C.; Laloi, M.; Cleusix, V.; Requena, T.; Meile, L.
    Hydrolysis of oligofructoses by the recombinant beta-fructofuranosidase from Bifidobacterium lactis (2004), Syst. Appl. Microbiol., 27, 279-285.
    View publication on PubMed
Search for more literature for 3.2.1.26

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 41% activation at 1 mM Bifidobacterium animalis subsp. lactis
DTT 26% activation at 1 mM Bifidobacterium animalis subsp. lactis
EDTA 32% activation at 1 mM Bifidobacterium animalis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
gene cscA, genetic organization, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue and as soluble, His-tagged enzyme in strain BL21(DE3) Bifidobacterium animalis subsp. lactis

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
Ca2+ 15% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
Co2+ 38% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
Cu2+ complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
Fe2+ 55% inhibition at 1 mM Bifidobacterium animalis subsp. lactis
SDS complete inhibition at 1 mM Bifidobacterium animalis subsp. lactis
Zn2+ 56% inhibition at 1 mM Bifidobacterium animalis subsp. lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Bifidobacterium animalis subsp. lactis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by 1 mM Mn2+ Bifidobacterium animalis subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, recombinant His-tagged enzyme, SDS-PAGE Bifidobacterium animalis subsp. lactis

Organism

Organism UniProt Comment Textmining
Bifidobacterium animalis subsp. lactis Q564C7 gene cscA, strain DSM 10140
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble, His-tagged CscA 238fold from Escherichia coli strain BL21(DE3) by metal affinity chromatography Bifidobacterium animalis subsp. lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bifidobacterium animalis subsp. lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan + H2O low activity Bifidobacterium animalis subsp. lactis D-fructose + ?
-
 ?
additional information no activity with melezitose, raffinose is a poor substrate Bifidobacterium animalis subsp. lactis ?
-
 ?
Raftiline HP + H2O low activity Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?
raftiline LS + H2O
-
 Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?
raftilose + H2O best substrate for CscA Bifidobacterium animalis subsp. lactis D-fructose
-
 ?
sucrose + H2O low activity Bifidobacterium animalis subsp. lactis D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, recombinant His-tagged enzyme, SDS-PAGE Bifidobacterium animalis subsp. lactis

Synonyms

Synonyms Comment Organism
beta-(1-2)-fructofuranosidase
-
 Bifidobacterium animalis subsp. lactis
CscA
-
 Bifidobacterium animalis subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bifidobacterium animalis subsp. lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Bifidobacterium animalis subsp. lactis