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Literature summary for 3.2.1.26 extracted from

  • Sanjay, G.; Sugunan, S.
    Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10 (2005), Food Chem., 94, 573-579.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilized on montmorillonite K-10 clay shows improved pH and thermal stabilities Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.1
-
sucrose free enzyme, pH 5.0, 30°C Saccharomyces cerevisiae
70.4
-
sucrose covalently bound enzyme, pH 5.0, 30°C Saccharomyces cerevisiae
130
-
sucrose adsorbed enzyme, pH 5.0, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Saccharomyces cerevisiae
-

Storage Stability

Storage Stability Organism
5°C, 0.1 M phosphate buffer, pH 5.0-6.0, the free enzyme loses all activity within 15 days. The immobilized enzyme shows no loss of activity up to 10 days, the adsorbed enzyme loses 50% of its activity in 30 days, the covalently bound enzyme loses 25% activity in 30 days Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
invertase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
free enzyme Saccharomyces cerevisiae
60
-
immobilized enzyme Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
temperature profiles of free and immobilized enzymes Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
free enzyme, stable up to Saccharomyces cerevisiae
60
-
free enzyme, stable up to Saccharomyces cerevisiae
80
-
inactivation of free enzyme Saccharomyces cerevisiae
90
-
inactivation of immobilized enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
free enzyme, and covalently bound enzyme Saccharomyces cerevisiae
6
-
adsorbed enzyme Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profiles of free and immobilized enzymes Saccharomyces cerevisiae
3 6.3 about 50% of maximal activity at pH 3.0 and pH 6.3, covalently bound enzyme Saccharomyces cerevisiae
3.7 7 about 50% of maximal activity at pH 3.7 and pH 7.0, free enzyme Saccharomyces cerevisiae
4 7.5 about 50% of maximal activity at pH 4.3 and pH 7.5, adsorbed enzyme Saccharomyces cerevisiae