Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilized on montmorillonite K-10 clay shows improved pH and thermal stabilities | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
sucrose | free enzyme, pH 5.0, 30°C | Saccharomyces cerevisiae | |
70.4 | - |
sucrose | covalently bound enzyme, pH 5.0, 30°C | Saccharomyces cerevisiae | |
130 | - |
sucrose | adsorbed enzyme, pH 5.0, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Saccharomyces cerevisiae | - |
Storage Stability | Organism |
---|---|
5°C, 0.1 M phosphate buffer, pH 5.0-6.0, the free enzyme loses all activity within 15 days. The immobilized enzyme shows no loss of activity up to 10 days, the adsorbed enzyme loses 50% of its activity in 30 days, the covalently bound enzyme loses 25% activity in 30 days | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
invertase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme | Saccharomyces cerevisiae |
60 | - |
immobilized enzyme | Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profiles of free and immobilized enzymes | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme, stable up to | Saccharomyces cerevisiae |
60 | - |
free enzyme, stable up to | Saccharomyces cerevisiae |
80 | - |
inactivation of free enzyme | Saccharomyces cerevisiae |
90 | - |
inactivation of immobilized enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
free enzyme, and covalently bound enzyme | Saccharomyces cerevisiae |
6 | - |
adsorbed enzyme | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profiles of free and immobilized enzymes | Saccharomyces cerevisiae |
3 | 6.3 | about 50% of maximal activity at pH 3.0 and pH 6.3, covalently bound enzyme | Saccharomyces cerevisiae |
3.7 | 7 | about 50% of maximal activity at pH 3.7 and pH 7.0, free enzyme | Saccharomyces cerevisiae |
4 | 7.5 | about 50% of maximal activity at pH 4.3 and pH 7.5, adsorbed enzyme | Saccharomyces cerevisiae |