KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
sucrose | pH 7.0, 37°C | Arthrobacter globiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, native enzyme, SDS-PAGE | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Arthrobacter globiformis IFO 3062 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 37fold by ammonium sulfate fractionation, dialysis, and two steps of ion exchange chromatography | Arthrobacter globiformis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
raffinose + H2O = beta-D-fructofuranose + 6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose | the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism | Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101 | - |
purified enzyme | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview | Arthrobacter globiformis | ? | - |
? | |
additional information | the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview | Arthrobacter globiformis IFO 3062 | ? | - |
? | |
sucrose + H2O | - |
Arthrobacter globiformis | D-glucose + D-fructose | - |
? | |
sucrose + H2O | - |
Arthrobacter globiformis IFO 3062 | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, native enzyme, SDS-PAGE | Arthrobacter globiformis |
Synonyms | Comment | Organism |
---|---|---|
beta-FFase | - |
Arthrobacter globiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Arthrobacter globiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, purified enzyme, over 90% remaining activity | Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
127 | - |
sucrose | pH 7.0, 37°C | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Arthrobacter globiformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | purified enzyme, over 90% of activity after 20 h at 4°C | Arthrobacter globiformis |