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Literature summary for 3.2.1.26 extracted from

  • Rubio, M.C.; Runco, R.; Navarro, A.R.
    Invertase from a strain of Rhodotorula glutinis (2002), Phytochemistry, 61, 605-609.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
227
-
sucrose pH 4.5, 35°C Rhodotorula glutinis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 3.4fold stimulation Rhodotorula glutinis
Co2+ 5 mM, 1.7fold stimulation Rhodotorula glutinis
Cu2+ 5 mM, 2fold stimulation Rhodotorula glutinis
Fe2+ 5 mM, 2.4fold stimulation Rhodotorula glutinis
Mg2+ 5 mM, 3.5fold stimulation Rhodotorula glutinis
Na+ 5 mM, 2fold stimulation Rhodotorula glutinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Rhodotorula glutinis
100000
-
gel filtration Rhodotorula glutinis

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 19% carbohydrate Rhodotorula glutinis

Purification (Commentary)

Purification (Comment) Organism
-
Rhodotorula glutinis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no transfructosylation reraction at 1 M sucrose Rhodotorula glutinis ?
-
?
raffinose + H2O
-
Rhodotorula glutinis beta-D-fructose + melibiose
-
?
stachyose + H2O
-
Rhodotorula glutinis ?
-
?
sucrose + H2O
-
Rhodotorula glutinis beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Rhodotorula glutinis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life is 30 min Rhodotorula glutinis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Rhodotorula glutinis

pH Range

pH Minimum pH Maximum Comment Organism
4 5 pH 4: about 70% of maximal activity, pH 5.0: about 60% of maximal activity Rhodotorula glutinis

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.6 5.5 stable Rhodotorula glutinis
6
-
30 min, 10% loss of activity Rhodotorula glutinis
8
-
30 min, 93% loss of activity Rhodotorula glutinis