Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.69 | - |
sucrose | pH 5.0, 37°C | Ipomoea batatas | |
32.19 | - |
raffinose | pH 5.0, 37°C | Ipomoea batatas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
1 * 68000, recombinant His-tagged enzyme | Ipomoea batatas |
72000 | - |
gel filtration | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | Q9ZTX2 | L. cv. Tainong 57 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains glucose and/or mannose. The carbohydrate moiety appears to be a key determinant of the enzymes sucrose hydrolysis activity, substrate affinity, and thermal stability | Ipomoea batatas |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Ipomoea batatas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ipomoea batatas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
raffinose + H2O | - |
Ipomoea batatas | beta-D-fructose + melibiose | - |
? | |
sucrose + H2O | - |
Ipomoea batatas | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, recombinant His-tagged enzyme | Ipomoea batatas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Ipomoea batatas |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
30 min, recombinant enzyme is stable deglycosylated enzyme loses 40% of the initial activity | Ipomoea batatas |
30 | - |
30 min, recombinant enzyme loses 20% of the initial activity, deglycosylated enzyme loses 60% of initial activity | Ipomoea batatas |
40 | - |
30 min, recombinant enzyme loses 50% of the initial activity, deglycosylated enzyme loses 90% of initial activity | Ipomoea batatas |
50 | - |
30 min, recombinant enzyme loses 80% of the initial activity, deglycosylated enzyme loses 95% of initial activity | Ipomoea batatas |
60 | - |
30 min, recombinant enzyme loses 85% of the initial activity, deglycosylated enzyme completely loses activity | Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Ipomoea batatas |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ipomoea batatas | - |
- |
4.9 |