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Literature summary for 3.2.1.26 extracted from
- Cloning, characterization and functional expression of a new beta-D-fructofuranosidase (Osbetafruct2) cDNA from Oryza sativa (2003), Biotechnol. Lett., 25, 455-459.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Oryza sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the amino acid sequence contains four potential N-linked glycosylation sites | Oryza sativa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme containing a 6*His-tagged tail | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | - |
Oryza sativa | beta-D-fructose + alpha-D-melibiose | - |
? |  |
| sucrose + H2O | - |
Oryza sativa | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Oryza sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oryza sativa | calculation from nucleotide sequence | - |
4.9 |
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