Cloned (Comment) | Organism |
---|---|
- |
Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
sucrose | pH 7.0, 30°C | Zymomonas mobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Zymomonas mobilis | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Zymomonas mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Zymomonas mobilis | Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form | beta-D-fructose + alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Zymomonas mobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
129 | - |
- |
Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | 1.8% of the activity with sucrose, isoenzyme AIV I and AIV II | Zymomonas mobilis | beta-D-fructose + ? | - |
? | |
raffinose + H2O | about 30% of the activity with sucrose, isoenzyme AIV I and AIV II | Zymomonas mobilis | beta-D-fructose + melibiose | - |
? | |
sucrose + H2O | - |
Zymomonas mobilis | beta-D-fructose + alpha-D-glucose | - |
? | |
sucrose + H2O | Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form | Zymomonas mobilis | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Zymomonas mobilis |
Synonyms | Comment | Organism |
---|---|---|
SacA | - |
Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Zymomonas mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 30 | active in the range | Zymomonas mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
30 min, 50% loss of activity | Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
125 | - |
sucrose | pH 7.0, 30°C | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | active in the range | Zymomonas mobilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zymomonas mobilis | electrofocusing | - |
7.1 |