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Literature summary for 3.2.1.26 extracted from

  • Nakagawa, H.; Arao, T.; Matsuzawa, T.; Ito, S.; Ogura, N.; Takehana, H.
    Comparison of the properties of tomato beta-fructofuranosidase embedded within a polyacrylamide gel and adsorbed on CM-cellulose (1975), Agric. Biol. Chem., 39, 1-5.
No PubMed abstract available
Search for more literature for 3.2.1.26

General Stability

General Stability Organism
enzyme embedded within a polyacrylamide gel and freeze dried retains only 25% of their original activity Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, 95% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 72.7% Solanum lycopersicum
Cu2+ CuSO4 Solanum lycopersicum
PCMB 0.1 mM, 94% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 16.7% Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 sucrose enzyme embedded within a polyacrylamide gel Solanum lycopersicum
6.9
-
 sucrose enzyme adsorbed on CM-cellulose Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
K4Fe(CN)6 activates enzyme embedded within a polyacrylamide gel, no effect on CM-cellulose-bound enzyme Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Solanum lycopersicum D-fructose + D-glucose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 5 min, complete inactivation of the CM-cellulose adsorbed enzyme Solanum lycopersicum
70
-
 5 min, enzyme embedded within a polyacrylamide gel loses 85% loss of activity Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 free enzyme and enzyme embedded within a polyacrylamide gel Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
3.3 6 pH 3.3: about 50% of maximal activity of the free enzyme, about 70% of maximal activity of the enzyme embedded within a polyacrylamide gel, pH 6.0: about 10% of activity of the free enzyme, about 45% of activity of the enzyme embedded within a polyacrylamide gel Solanum lycopersicum