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Literature summary for 3.2.1.25 extracted from
- Exchange of active site residues alters substrate specificity in extremely thermostable beta-glycosidase from Thermococcus kodakarensis KOD1 (2015), Enzyme Microb. Technol., 77, 14-20 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild type and mutant enzymes Q77R, D206N, D206Q, E207S and E399S are expressed in Escherichia coli | Thermococcus kodakarensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D206N | catalytically active mutant enzyme, similar temperature optimum like wild-type enzyme. The high-catalytic turn-over rate by D206N for beta-glucosidase activity makes it a useful enzyme in cellulose degradation at high temperatures | Thermococcus kodakarensis |
| D206Q | mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides | Thermococcus kodakarensis |
| E207S | no hydrolytic activity | Thermococcus kodakarensis |
| E399S | no hydrolytic activity | Thermococcus kodakarensis |
| Q77R | mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis |  |
| 0.94 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 1.08 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 1.29 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 4.6 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 9.23 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q9YGB8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.69 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 50.99 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 227.64 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 322.25 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 323.41 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 349.62 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.9 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 47.21 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 49.48 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
| 51.72 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 250.7 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 342.81 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH is not specified in the publication, wild-type | Thermococcus kodakarensis | |
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