Any feedback?
Literature summary for 3.2.1.25 extracted from
- Prokaryotic expression, purification and characterization of Aspergillus sulphureus beta-mannanase and site-directed mutagenesis of the catalytic residues (2008), Appl. Biochem. Biotechnol., 149, 139-144.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aspergillus sulphureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E206G | complete loss of activity | Aspergillus sulphureus |
| E314G | complete loss of activity | Aspergillus sulphureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus sulphureus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 208 | - |
50°C, pH 2.4 | Aspergillus sulphureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Aspergillus sulphureus | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus sulphureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, complete loss of activity | Aspergillus sulphureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
- |
Aspergillus sulphureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
