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Literature summary for 3.2.1.25 extracted from
- Cloning, sequence, expression and characterization of human beta-mannosidase (2008), Acta Biochim. Pol., 55, 479-490.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1.5 mM, no residual activity | Homo sapiens |  |
| Co2+ | 1.5 mM, 80% residual activity | Homo sapiens | |
| Cu2+ | 1.5 mM, no residual activity | Homo sapiens | |
| dimethyl sulfoxide | 5 mM, no residual activity | Homo sapiens | |
| dimethylformamide | 5 mM, no residual activity | Homo sapiens | |
| ethanol | 5 mM, no residual activity | Homo sapiens | |
| iodoacetate | 5 mM, no residual activity | Homo sapiens | |
| Pb2+ | 1.5 mM, 78% residual activity | Homo sapiens | |
| Sodium dodecyl sulfate | 5 mM, no residual activity | Homo sapiens | |
| Zn2+ | 1.5 mM, no residual activity | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | 3 | 4-nitrophenyl-beta-D-mannopyranoside | pH 5.0, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
x * 97000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | A7LFP5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | transcription in all cells and tissues tested | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-mannopyranose | - |
? | |
| additional information | no substrate: 4-nitrophenyl-alpha-D-mannose | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 97000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Homo sapiens |
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Release 2023.1 (January 2023)
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