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Literature summary for 3.2.1.25 extracted from

  • Dias, F.M.; Vincent, F.; Pell, G.; Prates, J.A.; Centeno, M.S.; Tailford, L.E.; Ferreira, L.M.; Fontes, C.M.; Davies, G.J.; Gilbert, H.J.
    Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A (2004), J. Biol. Chem., 279, 25517-25526.
    View publication on PubMed
Search for more literature for 3.2.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cellvibrio mixtus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Cellvibrio mixtus

Protein Variants

Protein Variants Comment Organism
E330A mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside Cellvibrio mixtus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000083
-
 4-nitrophenyl-beta-mannopyranose pH 7.0, 37°C, mutant enzyme E215A Cellvibrio mixtus
0.1
-
 Mannotriose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
0.9
-
 mannobiose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
1.6
-
 4-nitrophenyl-beta-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
6.1
-
 beta-D-mannosyl-1,4-beta-D-glucoside pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus

Organism

Organism UniProt Comment Textmining
Cellvibrio mixtus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Cellvibrio mixtus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-galactosyl-mannotriose + H2O
-
 Cellvibrio mixtus ?
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Cellvibrio mixtus 4-nitrophenol + alpha-D-mannose
-
 ?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
-
 Cellvibrio mixtus D-mannopyranose
-
 ?
beta-D-mannosyl-1,4-beta-D-glucoside + H2O
-
 Cellvibrio mixtus D-mannose + beta-D-glucose
-
 ?
mannobiose + H2O
-
 Cellvibrio mixtus 2 D-mannopyranose
-
 ?
mannotetraose + H2O
-
 Cellvibrio mixtus ?
-
 ?
mannotriose + H2O
-
 Cellvibrio mixtus ?
-
 ?
additional information CmMan5A releases beta-1,4-linked mannose substrates exclusively from the nonreducing end Cellvibrio mixtus ?
-
 ?

Synonyms

Synonyms Comment Organism
CmMan5a
-
 Cellvibrio mixtus
mannosidase 5A
-
 Cellvibrio mixtus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
 4-nitrophenyl-beta-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
0.053
-
 beta-D-mannosyl-1,4-beta-D-glucoside pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
0.1
-
 4-nitrophenyl-beta-mannopyranose pH 7.0, 37°C, mutant enzyme E215A Cellvibrio mixtus
2.17
-
 4-O-beta-D-mannopyranosyl-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
166.7
-
 Mannotriose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus