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Literature summary for 3.2.1.25 extracted from
- Mannan-degrading enzymes from Cellulumonas fimi (1999), Appl. Environ. Microbiol., 65, 2598-2605.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| man2A gene is subcloned into the expression vector pET28A(+), yielding the plasmid pET28Man. The protein encoded by this plasmid carries a C-terminal His6 tag for purification, expression in Escherichia coli BL21 | Cellulomonas fimi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-nitrophenyl-beta-mannoside | above 0.4 mM | Cellulomonas fimi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the KM-value of 0.3 mM is only an estimate | Cellulomonas fimi |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Cellulomonas fimi | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Cellulomonas fimi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas fimi | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolysis of p-nitrophenyl derivatives of alpha-mannose, beta-N-acetylglucosamine, beta-glucose, beta-xylose, beta-cellobiose or beta-gentiobiose | Cellulomonas fimi | ? | - |
? | |
| p-nitrophenyl-beta-galactoside + H2O | low activity | Cellulomonas fimi | p-nitrophenol + beta-D-galactopyranose | - |
? | |
| p-nitrophenyl-beta-mannoside + H2O | - |
Cellulomonas fimi | p-nitrophenol + beta-D-mannopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-mannosidase 2A | - |
Cellulomonas fimi |
| Man2A | - |
Cellulomonas fimi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Cellulomonas fimi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life: 27 h | Cellulomonas fimi |
| 55 | - |
unstable at | Cellulomonas fimi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate | Cellulomonas fimi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cellulomonas fimi |
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Release 2023.1 (January 2023)
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