Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.25 extracted from

  • Guadalupi, R.; Bernard, M.; Orlacchio, A.; Foglietti, M.J.; Emiliani, C.
    Purification and properties of human urinary beta-D-mannosidase (1996), Biochim. Biophys. Acta, 1293, 9-16.
    View publication on PubMed
Search for more literature for 3.2.1.25

General Stability

General Stability Organism
both forms of enzyme are partially stabilized by human albumin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 4-methylumbelliferyl beta-D-mannopyranoside beta-mannosidase B Homo sapiens
0.35
-
 4-methylumbelliferyl beta-D-mannopyranoside beta-mannosidase A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
49000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
49000
-
 beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens
75000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
75000
-
 beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens
135000
-
 beta-mannosidase A, enzyme from urine, nondenaturing gradient PAGE Homo sapiens
137000
-
 beta-mannosidase A, enzyme from kidney, gradient gel electrophoresis Homo sapiens
155000
-
 beta-mannosidase B, enzyme from kidney, gradient gel electrophoresis Homo sapiens
160000
-
 beta-mannosidase B, enzyme from urine, nondenaturing gradient PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two forms of enzyme: beta-mannosidase A and B Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.772
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-mannoside + H2O
-
 Homo sapiens 4-methylumbelliferone + beta-D-mannose
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
trimer beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 1 h, stable Homo sapiens
40
-
 1 h, stable Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
 Homo sapiens