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Literature summary for 3.2.1.25 extracted from

  • Sukeno, T.; Tarentino, A.L.; Plummer, T.H.; Maley, F.
    Purification and properties of alpha-D- and beta-D-mannosidases from hen oviduct (1972), Biochemistry, 11, 1493-1501.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride 0.6 M, 30% inhibition Gallus gallus
Hg2+ 0.05 mM, 50% inhibition Gallus gallus
p-nitrophenyl-alpha-D-mannopyranoside hydrolysis of p-nitrophenyl-beta-D-mannoside Gallus gallus
PCMB
-
Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
p-nitrophenyl-beta-D-mannoside
-
Gallus gallus
16.9
-
Asn-(GlcNAc)2(Man)
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.6
-
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asn-(GlcNAc)2(Man) + H2O
-
Gallus gallus ?
-
?
p-nitrophenyl-beta-D-mannoside + H2O
-
Gallus gallus p-nitrophenol + alpha-D-mannose
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
Hg2+
-
Gallus gallus