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Literature summary for 3.2.1.25 extracted from
- Purification and properties of beta-mannosidase from malted barley (1974), Biochim. Biophys. Acta, 370, 276-282.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| p-nitrophenyl-alpha-D-mannopyranoside | activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM | Hordeum vulgare |  |
General Stability
| General Stability | Organism |
|---|---|
| no inactivation by freezing and thawing | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-amino-2-deoxy-D-mannose | competitive | Hordeum vulgare | |
| p-nitrophenyl-alpha-D-mannopyranoside | activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM | Hordeum vulgare | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
p-nitrophenyl-beta-D-mannoside | - |
Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
x * 88000, SDS-PAGE | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 0.01% carbohydrate covalently bound to the partially purified enzyme | Hordeum vulgare |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.506 | - |
- |
Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, lyophilized enzyme, stable for over a year | Hordeum vulgare |
| 4°C, in aqueous solution, stable for several months | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-mannoside + H2O | p-nitrophenyl-beta-D-mannopyranoside | Hordeum vulgare | p-nitrophenol + alpha-D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Hordeum vulgare |
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Release 2023.1 (January 2023)
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