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Literature summary for 3.2.1.25 extracted from
- Purification and properties of a beta-mannosidase from Aspergillus niger (1977), J. Biol. Chem., 252, 2026-2031.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
p-nitrophenyl-beta-D-mannoside | about | Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus niger | locust bean gum and guar flour are the best substrates for enzyme formation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | oligosaccharide is attached to the protein in a GlcNAc-Asn linkage, mannose and glucosamine in the ratio of 2.5 or 3:1 | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man-(beta1-4)GlcNAc + H2O | - |
Aspergillus niger | mannose + GlcNAc | - |
? | |
| Man-(beta1-4)ManNAc + H2O | - |
Aspergillus niger | mannose + ManNAc | - |
? | |
| Man-GlcNAc-GlcNAc + H2O | - |
Aspergillus niger | mannose + GlcNAc-GlcNAc | - |
? | |
| additional information | locust bean gum and guar flour are the best substrates for enzyme formation | Aspergillus niger | ? | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | no activity with p-nitrophenyl-alpha-D-mannoside | Aspergillus niger | p-nitrophenol + alpha-D-mannose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4.5 | - |
Aspergillus niger |
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Release 2023.1 (January 2023)
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