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Literature summary for 3.2.1.25 extracted from

  • Bouquelet, S.; Spik, G.; Montreuil, J.
    Properties of a beta-D-mannosidase from Aspergillus niger (1978), Biochim. Biophys. Acta, 522, 521-530.
    View publication on PubMed
Search for more literature for 3.2.1.25

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Aspergillus niger
D-mannose with p-nitrophenyl-beta-D-mannopyranoside as substrate Aspergillus niger
PCMB
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 p-nitrophenyl-beta-D-mannopyranoside
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 equilibrium sedimentation Aspergillus niger
140000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein neutral sugar content: 17.8%. Presence of galactose, mannose and N-acetylglucosamine in a molar ratio of 1.3:7:1 Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
188
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-Asn-Lys + H2O
-
 Aspergillus niger ?
-
 ?
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + H2O
-
 Aspergillus niger beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + D-mannopyranose
-
 ?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
-
 Aspergillus niger mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
-
 ?
p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Aspergillus niger p-nitrophenol + alpha-D-mannose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 2 h, stable Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 hydrolysis of p-nitrophenyl-beta-D-mannopyranoside, at 55°C Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 7.5 37°C, 2 h, stable Aspergillus niger