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Literature summary for 3.2.1.212 extracted from

  • Silchenko, A.S.; Kusaykin, M.I.; Kurilenko, V.V.; Zakharenko, A.M.; Isakov, V.V.; Zaporozhets, T.S.; Gazha, A.K.; Zvyagintseva, T.N.
    Hydrolysis of fucoidan by fucoidanase isolated from the marine bacterium, Formosa algae (2013), Mar. Drugs, 11, 2413-2430 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.212

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.25 M Formosa algae
Zn2+ 0.25 M Formosa algae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 0.25 M, activates Formosa algae
Ca2+ 0.25 M, activates Formosa algae
Mg2+ 0.25 M, activates Formosa algae

Organism

Organism UniProt Comment Textmining
Formosa algae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Formosa algae

Source Tissue

Source Tissue Comment Organism Textmining
additional information the first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract is detected after 4 h of bacterial growth, and maximal fucoidanase activity is observed after 12 h of growth Formosa algae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fucoidan + H2O the enzyme catalyzes the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase does not hydrolyze carrageenan. Desulfated fucoidan from Fucus evanescens is hydrolysed very weakly in contrast to deacetylated fucoidan, which is hydrolysed more actively compared to the native fucoidan from Fucus evanescens. Analysis of the structure of the enzymatic products shows that the enzyme shows an endo-type action that is specific for (1->4)-bonds in a polysaccharide molecule built up of alternating 3- and 4-linked alpha-L-fucopyranose residues sulfated mainly at position 2 Formosa algae ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Formosa algae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 60 min, activity of fucoidanase is considerably reduced Formosa algae
55
-
 40 min, complete inactivation Formosa algae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9.1
-
 Formosa algae