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Literature summary for 3.2.1.212 extracted from

  • Silchenko, A.S.; Kusaykin, M.I.; Zakharenko, A.M.; Menshova, R.V.; Khanh, H.H.N.; Dmitrenok, P.S.; Isakov, V.V.; Zvyagintseva, T.N.
    Endo-1,4-fucoidanase from vietnamese marine mollusk Lambis sp. which producing sulphated fucooligosaccharides (2014), J. Mol. Catal. B, 102, 154-160 .
No PubMed abstract available
Search for more literature for 3.2.1.212

Activating Compound

Activating Compound Comment Organism Structure
Ba2+ 0.1 M, 1.1fold activation Lambis sp.
Ca2+ 0.1 M, 1.13fold activation Lambis sp.
Mg2+ 0.1 M, 1.08fold activation Lambis sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1 M, 75% loss of activity Lambis sp.
Hg2+ 0.1 M, 80% loss of activity Lambis sp.
Zn2+ 0.1 M, 55% loss of activity Lambis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE Lambis sp.

Organism

Organism UniProt Comment Textmining
Lambis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lambis sp.

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland
-
 Lambis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fucoidan + H2O the fucoidanase catalyses the hydrolysis of 1->3/1->4-alpha-L-fucans from Fucus evanescens and Fucus vesiculosus, but not 1->3-alpha-L-fucan from Saccharina cichorioides. It catalyzes the hydrolysis of 1->4-bonds in a fucoidan molecule as endo-enzyme Lambis sp. sulphated fucoidan oligosaccharides with different degree of polymerization
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Lambis sp.

Synonyms

Synonyms Comment Organism
endo-1,4-fucoidanase
-
 Lambis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Lambis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
 half-inactivation time: 20 min Lambis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
-
 Lambis sp.