Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose | the enzyme is activated by up to 250 mM D-glucose | Jeotgalibacillus malaysiensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Jeotgalibacillus malaysiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 61% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Co2+ | 4% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Cu2+ | complete inhibition at 5 mM | Jeotgalibacillus malaysiensis | |
D-glucose | the enzyme is able to tolerate D-glucose concentration up to 2500 mM, retaining about 80% of its maximal activity | Jeotgalibacillus malaysiensis | |
DMSO | 84% residual activity at 5% (v/v) | Jeotgalibacillus malaysiensis | |
EDTA | 4% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Fe3+ | 6% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Mn2+ | 7% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Ni2+ | 3% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Rb+ | 77% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
SDS | 2% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Triton X-100 | 70% residual activity at 5% (v/v) | Jeotgalibacillus malaysiensis | |
Tween 20 | 75% residual activity at 5% (v/v) | Jeotgalibacillus malaysiensis | |
Tween 80 | 47% residual activity at 5% (v/v) | Jeotgalibacillus malaysiensis | |
Urea | 85% residual activity at 5 mM | Jeotgalibacillus malaysiensis | |
Zn2+ | 7% residual activity at 5 mM | Jeotgalibacillus malaysiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 7.4 and 65°C | Jeotgalibacillus malaysiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 121% activity at 5 mM. The activity and stability are increased when CaCl2 is supplemented to the enzyme | Jeotgalibacillus malaysiensis | |
additional information | not influenced by Na+, K+ and Mg2+ | Jeotgalibacillus malaysiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Jeotgalibacillus malaysiensis | - |
- |
- |
Jeotgalibacillus malaysiensis DSM 28777T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Jeotgalibacillus malaysiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Jeotgalibacillus malaysiensis | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Jeotgalibacillus malaysiensis DSM 28777T | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
cellobiose + H2O | 100% activity | Jeotgalibacillus malaysiensis | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | 100% activity | Jeotgalibacillus malaysiensis DSM 28777T | 2 beta-D-glucose | - |
? | |
cellohexanose + H2O | 85% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + ? | - |
? | |
cellopentose + H2O | 125% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + ? | - |
? | |
cellotetraose + H2O | 125% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + ? | - |
? | |
cellotetraose + H2O | 125% activity compared to cellobiose | Jeotgalibacillus malaysiensis DSM 28777T | beta-D-glucose + ? | - |
? | |
cellotriose + H2O | 124% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + ? | - |
? | |
gentibiose + H2O | 54% activity compared to cellobiose | Jeotgalibacillus malaysiensis | 2 beta-D-glucose | - |
? | |
lactose + H2O | 34% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + D-galactose | - |
? | |
additional information | not active towards sucrose and maltose as well as polysaccharides such as Avicel, carboxymethyl cellulose, Sigmacell cellulose 101, alpha-cellulose and xylan | Jeotgalibacillus malaysiensis | ? | - |
? | |
additional information | not active towards sucrose and maltose as well as polysaccharides such as Avicel, carboxymethyl cellulose, Sigmacell cellulose 101, alpha-cellulose and xylan | Jeotgalibacillus malaysiensis DSM 28777T | ? | - |
? | |
salicin + H2O | 118% activity compared to cellobiose | Jeotgalibacillus malaysiensis | beta-D-glucose + ? | - |
? | |
salicin + H2O | 118% activity compared to cellobiose | Jeotgalibacillus malaysiensis DSM 28777T | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Jeotgalibacillus malaysiensis |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucosidase | - |
Jeotgalibacillus malaysiensis |
Bgl | - |
Jeotgalibacillus malaysiensis |
BglD5 | - |
Jeotgalibacillus malaysiensis |
cellobiase | - |
Jeotgalibacillus malaysiensis |
gentiobiase | - |
Jeotgalibacillus malaysiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Jeotgalibacillus malaysiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | more than 50% activity between 50 and 75°C | Jeotgalibacillus malaysiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the half-life at 65°C without Ca2+ is 35 min, the half-life at 65°C wit Ca2+ is 70 min | Jeotgalibacillus malaysiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.93 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 7.4 and 65°C | Jeotgalibacillus malaysiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in sodium phosphate buffer | Jeotgalibacillus malaysiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | more than 85% activity at pH 6.0-7.5 | Jeotgalibacillus malaysiensis |