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Literature summary for 3.2.1.21 extracted from

  • Bonfa, E.; de Souza Moretti, M.; Gomes, E.; Bonilla-Rodriguez, G.
    Biochemical characterization of an isolated 50kDa beta-glucosidase from the thermophilic fungus Myceliophthora thermophila M.7.7 (2018), Biocatal. Agricult. Biotechnol., 13, 311-318 .
No PubMed abstract available
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
4-hydroxybenzoic acid 109.7% activity at 2.5 mM with cellobioside as substrate Thermothelomyces thermophilus
p-coumaric acid 118% activity at 2.5 mM with cellobioside as substrate Thermothelomyces thermophilus
syringic acid 111.4% activity at 2.5 mM with cellobioside as substrate Thermothelomyces thermophilus
vanillic acid 125.1% activity at 2.5 mM with cellobioside as substrate Thermothelomyces thermophilus
Vanillin 118% activity at 2.5 mM with cellobioside as substrate Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzoic acid 97.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
acetone 68.3% and 62% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Al3+ 83.5% and 43.7% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Ba2+ 76.7% residual activity at 2.5 mM with cellobiose as substrate Thermothelomyces thermophilus
Cu2+ complete inhibition at 2.5 mM Thermothelomyces thermophilus
D-glucose competitive inhibition Thermothelomyces thermophilus
dithiothreitol 82.5% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
DMSO 92.9% and 57% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
ethanol 70.6% and 55.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Fe2+ complete inhibition at 2.5 mM Thermothelomyces thermophilus
ferulic acid 97.4% and 99.1% residual activity at 0.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
gallic acid 91.1% and 75.3% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Isopropanol 66.5-70.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
K+ 76.2% and 81.9% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Mg2+ 62.7% residual activity at 2.5 mM with cellobiose as substrate Thermothelomyces thermophilus
Mn2+ 49.6% and 67.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
Na+ 70.6% and 57.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
p-coumaric acid 70.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
PEG 8000 67.6% and 58.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
polyvinyl alcohol 71.2% and 56.8% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
SDS 82.4% and 60.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
syringaldehyde 92.8% and 96.4% residual activity at 0.25 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
syringic acid 95.2% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
Tannic acid complete inhibition at 0.0625 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
Triton X-100 57.5% and 45% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus
vanillic acid 87.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
Vanillin 94% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus
Zn2+ 63% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 4-nitrophenyl beta-D-glucopyranoside at pH 5.0 and 60°C Thermothelomyces thermophilus
8.5
-
 cellobiose at pH 5.0 and 50°C Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 102.6-128.4% activity at 2.5 mM Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus
-
-
-
Thermothelomyces thermophilus M.7.7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose resin column chromatography and Sephacrly S-100 gel filtration Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus M.7.7 4-nitrophenol + beta-D-glucopyranose
-
 ?
cellobiose + H2O
-
 Thermothelomyces thermophilus 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Thermothelomyces thermophilus M.7.7 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
BGL50
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 with cellobiose as substrate Thermothelomyces thermophilus
60
-
 with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme is very stable at 60°C displaying a half-life of 855.6 min but it is easily denatured above this temperature Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 with cellobiose as substrate Thermothelomyces thermophilus
5
-
 with 4-nitrophenyl beta-D-glucopyranoside as substrate Thermothelomyces thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
 D-glucose at pH 5.0 and 60°C Thermothelomyces thermophilus