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Literature summary for 3.2.1.21 extracted from
- Understanding the role of residues around the active site tunnel towards generating a glucose-tolerant beta-glucosidase from Agrobacterium tumefaciens 5A (2017), Protein Eng. Des. Sel., 30, 523-530 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Agrobacterium tumefaciens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure | Agrobacterium tumefaciens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C174V | contrary to uncompetitive inhibition of wild-type by glucose, mutant is competitively inhibited | Agrobacterium tumefaciens |
| H229S | contrary to uncompetitive inhibition of wild-type by glucose, mutant is competitively inhibited | Agrobacterium tumefaciens |
| L178E | mutant shows enhanced thermostability | Agrobacterium tumefaciens |
| L178E | specific activity is1.8 times increased on substrate cellobiose | Agrobacterium tumefaciens |
| V176A | slight increase in specific activity | Agrobacterium tumefaciens |
| W127F | mutant shows enhanced thermostability | Agrobacterium tumefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | - |
Agrobacterium tumefaciens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.85 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 2.61 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 2.7 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 2.9 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 3.1 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 5.5 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 6.8 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 7 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 8 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 8.3 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 8.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 10.1 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 10.14 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 12.5 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 13.3 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 19.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 22.4 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | H0HC94 | - |
- |
| Agrobacterium tumefaciens 5A | H0HC94 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 204 | - |
wild-type, substrate cellobiose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
| 248 | - |
wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens 5A | nitrophenol + D-glucose | - |
? | |
| cellobiose + H2O | - |
Agrobacterium tumefaciens | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Agrobacterium tumefaciens 5A | 2 D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AT5A_15831 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
mutant C174W | Agrobacterium tumefaciens |
| 52 | - |
wild-type, mutants V176A, L178A, H229S | Agrobacterium tumefaciens |
| 53 | - |
mutant L178E | Agrobacterium tumefaciens |
| 55 | - |
mutant W127F | Agrobacterium tumefaciens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 91 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 95 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 107 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 118 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 131.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 163 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 193 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 230 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 233 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 277 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 282 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 324 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 328 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 334.9 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 354 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 363 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 390 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 559 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
mutant W127F | Agrobacterium tumefaciens |
| 7 | - |
mutants C174V, V176A, L178A, L178E, H229S | Agrobacterium tumefaciens |
| 7.2 | - |
wild-type | Agrobacterium tumefaciens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 518 | - |
D-glucose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 558 | - |
D-glucose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 558 | - |
D-glucose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 666 | - |
D-glucose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 686 | - |
D-glucose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.5 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 14.5 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 15.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 21.4 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 22.7 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 23.3 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 25.2 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 28.3 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 28.5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 36.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 39.7 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 41.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 45.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 49.4 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 70.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 78.2 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 79.3 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
| 89.9 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
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