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Literature summary for 3.2.1.21 extracted from
- Characterization of a metagenome-derived beta-glucosidase and its application in conversion of polydatin to resveratrol (2016), Catalysts, 6, 35 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | unidentified microorganism |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure. Residues Asp333 and Glu530 may act as the catalytic nucleophile and acid/base, respectively. Residues Val87, Leu187, Thr368, and Trp474 may participate in substrate recognition and binding | unidentified microorganism |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 44% residual activity | unidentified microorganism |  |
| Ca2+ | 1 mM, 58% residual activity | unidentified microorganism | |
| Cu2+ | 1 mM, 8% residual activity | unidentified microorganism | |
| EDTA | 1 mM, 56% residual activity | unidentified microorganism | |
| SDS | 1 mM, 76% residual activity | unidentified microorganism | |
| Zn2+ | 1 mM, 12% residual activity | unidentified microorganism | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| unidentified microorganism | - |
metagenomic library of mangrove soil | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 127 | - |
substrate esculin, pH 7.0, 40°C | unidentified microorganism |
| 248 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 7.0, 40°C | unidentified microorganism |
| 422 | - |
substrate polydatin, pH 7.0, 40°C | unidentified microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
unidentified microorganism | 4-nitrophenol + beta-D-glucose | - |
? | |
| esculin + H2O | - |
unidentified microorganism | beta-D-glucose + esculetin | - |
? | |
| additional information | enzyme exhibits weak activities toward 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl beta-D-xylopyranoside, but no detectable activity toward 4-nitrophenyl beta-D-cellobioside | unidentified microorganism | ? | - |
? | |
| polydatin + H2O | - |
unidentified microorganism | D-glucose + 3,4',5-trihydroxystilbene | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, SDS-PAGE, recombinant protein | unidentified microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
unidentified microorganism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | more than 50% of maximum activity | unidentified microorganism |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 989 | - |
polydatin | pH 7.0, 40°C | unidentified microorganism | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
unidentified microorganism |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1476 | - |
polydatin | pH 7.0, 40°C | unidentified microorganism | |
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