Literature summary for 3.2.1.21 extracted from

Tiwari, R.; Singh, P.; Singh, S.; Nain, P.; Nain, L.; Shukla, P.
Bioprospecting of novel thermostable beta-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis (2017), Biotechnol. Biofuels, 10, 246 .

Search for more literature for 3.2.1.21

Application

Application	Comment	Organism
degradation	saccharification of pretreated paddy straw by supplementing beta-glucosidase enzyme results in 1.34fold higher glucose release	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure based on PDB entry 2XHY	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	1 M, 70% residual activity	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.9	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.0, temperature not specified in the publication	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Bacillus subtilis RA10	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
47.9	-	pH 5.0, temperature not specified in the publication	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Bacillus subtilis	4-nitrophenol + beta-D-glucose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Bacillus subtilis RA10	4-nitrophenol + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
?	x * 57130, calculated, x * 57000, SDS-PAGE	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
80	-	78% of maximum activity	Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	48 h, 68% residual activity	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.0, temperature not specified in the publication	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Bacillus subtilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	-	70% of maximum activity	Bacillus subtilis

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Release 2023.1 (January 2023)