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Literature summary for 3.2.1.21 extracted from

  • Hassan, N.; Geiger, B.; Gandini, R.; Patel, B.; Kittl, R.; Haltrich, D.; Nguyen, T.; Divne, C.; Tan, T.
    Engineering a thermostable Halothermothrix orenii beta-glucosidase for improved galacto-oligosaccharide synthesis (2016), Appl. Microbiol. Biotechnol., 100, 3533-3543 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Protein Variants

Protein Variants Comment Organism
F417S significantly impaired catalytic activity with cellobiose and lactose as substrates, 1000fold decrease in apparent kcat of cellobiose, 47fold decrease in kcat of lactose. Mutant produces improved total galactooligosaccharides yields by transgalactosylation Halothermothrix orenii
F417Y mutant retains the capacity to hydrolyze both cellobiose and lactose with the same relative substrate selectivity as the wild-type, but with about 10fold lower turnover numbers. Mutant produces improved total galactooligosaccharides yields by transgalactosylation Halothermothrix orenii
N222F significantly impaired catalytic activity with cellobiose and lactose as substrates Halothermothrix orenii
N294T significantly impaired catalytic activity with cellobiose and lactose as substrates Halothermothrix orenii
Y296F significantly impaired catalytic activity with cellobiose and lactose as substrates. Mutant produces improved total galactooligosaccharides yields by transgalactosylation Halothermothrix orenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
 cellobiose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
9
-
 cellobiose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
10
-
 cellobiose wild-type, pH 7.0, 70°C Halothermothrix orenii
10.3
-
 lactose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
11.9
-
 cellobiose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
20.2
-
 cellobiose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
23.2
-
 lactose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
26.3
-
 lactose wild-type, pH 7.0, 70°C Halothermothrix orenii
29.1
-
 lactose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
30.8
-
 lactose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
41.3
-
 lactose mutant N222F, pH 7.0, 70°C Halothermothrix orenii
93.2
-
 cellobiose mutant N222F, pH 7.0, 70°C Halothermothrix orenii

Organism

Organism UniProt Comment Textmining
Halothermothrix orenii B8CYA8
-
-
Halothermothrix orenii DSM 9562 B8CYA8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
 Halothermothrix orenii 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Halothermothrix orenii DSM 9562 2 beta-D-glucose
-
 ?
lactose + H2O
-
 Halothermothrix orenii D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Halothermothrix orenii DSM 9562 D-glucose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
BglA
-
 Halothermothrix orenii
Hore_15280
-
 Halothermothrix orenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 cellobiose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
1.47
-
 lactose mutant N222F, pH 7.0, 70°C Halothermothrix orenii
6.9
-
 lactose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
7.4
-
 lactose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
7.8
-
 lactose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
8.5
-
 cellobiose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
16.5
-
 cellobiose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
16.9
-
 cellobiose mutant N222F, pH 7.0, 70°C Halothermothrix orenii
38.7
-
 cellobiose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
41.5
-
 lactose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
366
-
 lactose wild-type, pH 7.0, 70°C Halothermothrix orenii
387
-
 cellobiose wild-type, pH 7.0, 70°C Halothermothrix orenii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.032
-
 cellobiose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
0.036
-
 lactose mutant N222F, pH 7.0, 70°C Halothermothrix orenii
0.18
-
 cellobiose mutant N222F, pH 7.0, 70°C Halothermothrix orenii
0.24
-
 lactose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
0.24
-
 lactose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
0.34
-
 lactose mutant F417S, pH 7.0, 70°C Halothermothrix orenii
0.42
-
 cellobiose mutant Y296F, pH 7.0, 70°C Halothermothrix orenii
1.8
-
 cellobiose mutant N294T, pH 7.0, 70°C Halothermothrix orenii
4
-
 lactose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
11.1
-
 cellobiose mutant F417Y, pH 7.0, 70°C Halothermothrix orenii
13.9
-
 lactose wild-type, pH 7.0, 70°C Halothermothrix orenii
38.7
-
 cellobiose wild-type, pH 7.0, 70°C Halothermothrix orenii