Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Thermomyces lanuginosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive | Thermomyces lanuginosus | |
D-glucose | competitive | Thermomyces lanuginosus | |
Hg2+ | 2 mM, complete inhibition | Thermomyces lanuginosus | |
N-bromosuccinimide | 2 mM, complete inhibition | Thermomyces lanuginosus | |
Salicin | competitive | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 50°C | Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Thermomyces lanuginosus SSBP | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
substrate cellobiose, pH 6.5, 50°C | Thermomyces lanuginosus |
9.65 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 50°C | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces lanuginosus | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces lanuginosus SSBP | 4-nitrophenol + D-glucose | - |
? | |
cellobiose + H2O | - |
Thermomyces lanuginosus | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Thermomyces lanuginosus SSBP | 2 D-glucose | - |
? | |
additional information | enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose | Thermomyces lanuginosus | ? | - |
? | |
additional information | enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose | Thermomyces lanuginosus SSBP | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 105000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable | Thermomyces lanuginosus |
70 | - |
30 min, complete loss of activity | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 50°C | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
stable when the pH of the storage buffer is above 5.0 | Thermomyces lanuginosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
cellobiose | pH 6.5, 50°C | Thermomyces lanuginosus | |
0.55 | - |
D-glucose | pH 6.5, 50°C | Thermomyces lanuginosus | |
0.81 | - |
Salicin | pH 6.5, 50°C | Thermomyces lanuginosus |