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Literature summary for 3.2.1.21 extracted from
- Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus (2001), Biochem. J., 353, 117-127 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-delta-gluconolactone | competitive | Thermoascus aurantiacus |  |
| D-glucose | competitive | Thermoascus aurantiacus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.114 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.21 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.25 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.272 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.434 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.637 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 1.34 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 350000 | - |
gel filtration | Thermoascus aurantiacus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
| isopropanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
| Methanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
| Thermoascus aurantiacus IMI 216529 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoascus aurantiacus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | 2-nitrophenol + D-glucose | - |
? | |
| 2-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2-nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus IMI 216529 | 4-nitrophenol + D-glucose | - |
? | |
| beta-beta-trehalose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2 D-glucose | - |
? | |
| gentiobiose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
| additional information | enzyme hydrolyses aryl and alkyl beta-D-glucosides and cello-oligosaccharides, and is specific for substrates with a beta-glycosidic linkage. The hydroxy groups at positions 2, 4 and 6 of a glucose residue at the non-reducing end of a disaccharide are essential for catalysis. Enzyme releases one glucose unit at a time from the non-reducing end of cello-oligosaccharides, and the rate of hydrolysis decreases with an increase in chain length. The enzyme catalyses the synthesis of methyl, ethyl and propyl beta-D-glucosides in the presence of methanol, ethanol and propan-2-ol respectively with either glucose or cellobiose, cellobiose is preferred | Thermoascus aurantiacus | ? | - |
? | |
| additional information | enzyme hydrolyses aryl and alkyl beta-D-glucosides and cello-oligosaccharides, and is specific for substrates with a beta-glycosidic linkage. The hydroxy groups at positions 2, 4 and 6 of a glucose residue at the non-reducing end of a disaccharide are essential for catalysis. Enzyme releases one glucose unit at a time from the non-reducing end of cello-oligosaccharides, and the rate of hydrolysis decreases with an increase in chain length. The enzyme catalyses the synthesis of methyl, ethyl and propyl beta-D-glucosides in the presence of methanol, ethanol and propan-2-ol respectively with either glucose or cellobiose, cellobiose is preferred | Thermoascus aurantiacus IMI 216529 | ? | - |
? | |
| n-octyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | n-octanol + D-glucose | - |
? | |
| sophorose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
| sophorose + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 120000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermoascus aurantiacus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
70% of maximum activity | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable for 48 h | Thermoascus aurantiacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 136 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus | |
| 141 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 235 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 235 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 242 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 284 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 284 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Thermoascus aurantiacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 5 | - |
Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
stable for 48 h | Thermoascus aurantiacus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
D-delta-gluconolactone | pH 5, 50°C | Thermoascus aurantiacus | |
| 0.29 | - |
D-glucose | pH 5, 50°C | Thermoascus aurantiacus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 102 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus | |
| 446 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 655 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus | |
| 684 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
| 864 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 940 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
| 2130 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
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