KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 70°C | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
gel filtration | Thermoascus aurantiacus |
89000 | - |
1 * 89000, SDS-PAGE | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 33% carbohydrate | Thermoascus aurantiacus |
Purification (Comment) | Organism |
---|---|
- |
Thermoascus aurantiacus |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-20°C, stable for at least a year | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + D-glucopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 89000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, stable | Thermoascus aurantiacus |
75 | - |
1 h, about 30% loss of activity | Thermoascus aurantiacus |
80 | - |
1 h, complete loss of activity | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | 4.0: about 50% of maximal activity, pH 6.0: about 35% of maximal activity | Thermoascus aurantiacus |