Cloned (Comment) | Organism |
---|---|
gene bglA, recombinant expression in Escherichia coli strain BL21 Codon-Plus | Thermotoga petrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-propanol | 40% v/v, almost complete inhibition | Thermotoga petrophila | |
Cu2+ | 20% inhibition at 1 mM | Thermotoga petrophila | |
ethanol | 40% v/v, almost complete inhibition | Thermotoga petrophila | |
Hg2+ | 90% inhibition at 1 mM | Thermotoga petrophila | |
methanol | 40% v/v, almost complete inhibition | Thermotoga petrophila | |
additional information | the enzyme is not inhibited by glucose up to 5% w/v | Thermotoga petrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 7.0, 90°C | Thermotoga petrophila | |
4.3 | - |
4-nitrophenyl-beta-D-cellobioside | pH 7.0, 90°C | Thermotoga petrophila | |
25 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 7.0, 90°C | Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51500 | - |
x * 51500 SDS-PAGE | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A5IL97 | gene bglA | - |
Thermotoga petrophila RKU-1 | A5IL97 | gene bglA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 Codon-Plus | Thermotoga petrophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
purified recombinant enzyme, substrate cellobiose, pH 7.0, 90°C | Thermotoga petrophila |
1520 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 90°C | Thermotoga petrophila |
9400 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-cellobioside, pH 7.0, 90°C | Thermotoga petrophila |
14000 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-galactopyranoside, pH 7.0, 90°C | Thermotoga petrophila |
29200 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-fucopyranoside, pH 7.0, 90°C | Thermotoga petrophila |
30400 | - |
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 7.0, 90°C | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermotoga petrophila RKU-1 | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga petrophila RKU-1 | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermotoga petrophila RKU-1 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila RKU-1 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-xylopyranose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila RKU-1 | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51500 SDS-PAGE | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
BglA | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | - |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | activity range, profile overview | Thermotoga petrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5300 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 7.0, 90°C | Thermotoga petrophila | |
17500 | - |
4-nitrophenyl-beta-D-cellobioside | pH 7.0, 90°C | Thermotoga petrophila | |
87400 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 7.0, 90°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, profile overview | Thermotoga petrophila |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme-substrate docking studies, overview | Thermotoga petrophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 7.0, 90°C | Thermotoga petrophila | |
4100 | - |
4-nitrophenyl-beta-D-cellobioside | pH 7.0, 90°C | Thermotoga petrophila | |
30800 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 7.0, 90°C | Thermotoga petrophila |