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Literature summary for 3.2.1.21 extracted from

  • Li, B.; Wang, Z.; Li, S.; Donelan, W.; Wang, X.; Cui, T.; Tang, D.
    Preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase from Pyrococcus furiosus (2013), BMC Biotechnol., 13, 73.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Application

Application Comment Organism
synthesis preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
cloned in frame with the Saccharomyces cerecisiae a-factor secretory signal and expressed in Pichia pastoris strain X-33 Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 22% inhibition, not significantly stimulated or inhibited by other divalent cations Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 dimer Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
694.6
-
 pH and temperature not specified in the publication Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 110 70°C: about 45% of maximal activity, 110°C: about 30% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 120 the enzyme retains more than 80% of its maximum activity from 30°C to 120°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 60% of maximal activity, pH 7.0: about 80% of maximal activity Pyrococcus furiosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 37°C, 1 h incubation, the enzyme retains more than 80% of its maximum activity Pyrococcus furiosus

pI Value

Organism Comment pI Value Maximum pI Value
Pyrococcus furiosus
-
-
 4.4