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Literature summary for 3.2.1.21 extracted from

  • An, D.S.; Cui, C.H.; Lee, H.G.; Wang, L.; Kim, S.C.; Lee, S.T.; Jin, F.; Yu, H.; Chin, Y.W.; Lee, H.K.; Im, W.T.; Kim, S.G.
    Identification and characterization of a novel Terrabacter ginsenosidimutans sp. nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV (2010), Appl. Environ. Microbiol., 76, 5827-5836.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-tagged fusion protein Terrabacter ginsenosidimutans

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ significant inhibition Terrabacter ginsenosidimutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol pH 7.0, 37°C Terrabacter ginsenosidimutans
4.2
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 37°C Terrabacter ginsenosidimutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE Terrabacter ginsenosidimutans
193000
-
 native protein, gel filtration Terrabacter ginsenosidimutans

Organism

Organism UniProt Comment Textmining
Terrabacter ginsenosidimutans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a glutathione-Sepharose 4B column Terrabacter ginsenosidimutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 2-nitrophenol + D-glucopyranose
-
 ?
3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 2 H2O Conversion of ginsenoside Rb1 to gypenoside LXXV. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 2 D-glucose
-
 ?
3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 3 H2O Conversion of ginsenoside Rb1 to C-K. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol + 3 D-glucose
-
 ?
3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + H2O Conversion of ginsenoside Rb1 to gypenoside XVII. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 is cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 3-O-beta-D-glucopyranosyl-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 4-nitrophenol + D-glucopyranose
-
 ?

Subunits

Subunits Comment Organism
trimer SDS-PAGE, 3 * 70000 Terrabacter ginsenosidimutans

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
 Terrabacter ginsenosidimutans
bgpA
-
 Terrabacter ginsenosidimutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Terrabacter ginsenosidimutans
45
-
 optimal temperature Terrabacter ginsenosidimutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 45 enzyme is stable at temperatures lower than 37°C, and about 32% of the activity is lost after incubation at 45°C for 30 min Terrabacter ginsenosidimutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, optimum pH Terrabacter ginsenosidimutans

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Terrabacter ginsenosidimutans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 no activity Terrabacter ginsenosidimutans