Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
4.84 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
6.05 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48660 | - |
deduced from cDNA | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q0GA07 | - |
- |
Purification (Comment) | Organism |
---|---|
using gel filtration and ion-exchange chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-lactopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-lactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Thermus thermophilus |
KNOUC202beta-gly | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | Thermus thermophilus |
90 | - |
optimal temperature | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
enzyme shows high thermostability not losing its activity at 80°C for 2 h in 0.05 M Na-phosphate buffer of pH 6.8 with Tm of 100.0°C in 0.02 M Tris-HCl buffer of pH 8.2 | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
167.4 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
288.3 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
optimal pH | Thermus thermophilus |
7 | - |
assay at | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34.6 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
47.7 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
700.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus |