Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Concanavalin A-Sepharose column chromatography, Shodex-phenyl HIC-814 column chromatography, and Sephacryl S-200 gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
crude extract, at pH 5.5 and 50°C | Arabidopsis thaliana |
148.5 | - |
after 164.5fold purification, at pH 5.5 and 50°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | 83% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Arabidopsis thaliana | 2-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 100% activity | Arabidopsis thaliana | 4-nitrophenol + D-glucopyranose | - |
? | |
additional information | the enzyme does not hydrolyze 4-nitrophenyl-beta-D-galactoside, cellobiose, D-salicin, and galacturonic acid | Arabidopsis thaliana | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | about 50% activity at 60°C, about 30% activity at 70°C, about 10% activity at 80°C, no activity at 90°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | senescence-induced loss in photosynthesis enhances cell wall beta-glucosidase activity | up |