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Literature summary for 3.2.1.21 extracted from

  • Su, J.; Xu, J.; Yu, H.; He, Y.; Lu, W.; Lin, G.
    Properties of a novel beta-glucosidase from Fusarium proliferatum ECU2042 that converts ginsenoside Rg3 into Rh2 (2009), J. Mol. Catal. B, 57, 278-283.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 73% residual activity at 10 mM Fusarium proliferatum
EDTA 67% residual activity at 10 mM Fusarium proliferatum
Mg2+ 75% residual activity at 10 mM Fusarium proliferatum
Mn2+ 77% residual activity at 10 mM Fusarium proliferatum
Ni2+ 74% residual activity at 10 mM Fusarium proliferatum
Zn2+ 92% residual activity at 10 mM Fusarium proliferatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.37
-
ginsenoside Rg3 in NaAc/HAc buffer (0.2 M, pH 5.0), at 50°C Fusarium proliferatum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 114% relative activity at 10 mM Fusarium proliferatum
additional information the enzyme is not apparently affected by heavy metal salts, such as Cu2+ and Fe3+ Fusarium proliferatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39100
-
2 * 39100, SDS-PAGE Fusarium proliferatum
78700
-
gel filtration Fusarium proliferatum

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum
-
-
-
Fusarium proliferatum ECU2042
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, butyl-Toyopearl 650M column chromatography, DEAE-Toyopearl 650M column chromatography, TSK gel filtration Fusarium proliferatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000081
-
crude extract, at pH 5.0 and 50°C Fusarium proliferatum
0.041
-
after 506fold purification, at pH 5.0 and 50°C Fusarium proliferatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorophenyl beta-D-glucopyranoside + H2O 227% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
4-chlorophenyl beta-D-glucopyranoside + H2O 227% activity compared to ginsenoside Rg3 Fusarium proliferatum ECU2042 ?
-
?
4-hydroxybenzyl beta-D-glucopyranoside + H2O 551% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
4-hydroxybenzyl beta-D-glucopyranoside + H2O 551% activity compared to ginsenoside Rg3 Fusarium proliferatum ECU2042 ?
-
?
4-methoxybenzyl beta-D-glucopyranoside + H2O 73.2% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
4-methoxybenzyl beta-D-glucopyranoside + H2O 73.2% activity compared to ginsenoside Rg3 Fusarium proliferatum ECU2042 ?
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O very low activity Fusarium proliferatum 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O very low activity Fusarium proliferatum ECU2042 4-nitrophenol + D-glucopyranose
-
?
barbaloin + H2O 63% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
butyl beta-D-glucopyranoside + H2O 39% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
cyclopentyl beta-D-glucopyranoside + H2O 65.9% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
esculin hydrate + H2O 31.7% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
ethyl beta-D-glucopyranoside + H2O 14.6% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
ginsenoside Rg3 + H2O 100% activity, the enzyme hydrolyzes the 3-C, beta-(1-2)-glucoside of ginsenoside Rg3 to produce ginsenoside Rh2, but does not sequentially hydrolyze the beta-D-glucosidic bond of ginsenoside Rh2 Fusarium proliferatum ginsenoside Rh2 + D-glucose
-
?
hexyl beta-D-glucopyranoside + H2O 53.7% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
isopropyl beta-D-glucopyranoside + H2O 9.8% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
additional information the purified enzyme exhibits almost no activity towards cellobiose and 4-nitrophenyl-beta-D-galactopyranoside, the enzyme does not hydrolyze 4-bromophenyl beta-D-glucopyranoside and ginsenoside Rh2 after incubation at 50°C for 24 h Fusarium proliferatum ?
-
?
additional information the purified enzyme exhibits almost no activity towards cellobiose and 4-nitrophenyl-beta-D-galactopyranoside, the enzyme does not hydrolyze 4-bromophenyl beta-D-glucopyranoside and ginsenoside Rh2 after incubation at 50°C for 24 h Fusarium proliferatum ECU2042 ?
-
?
propenyl beta-D-glucopyranoside + H2O 26.8% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
propyl beta-D-glucopyranoside + H2O 31.7% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?
salidroside + H2O 51.2% activity compared to ginsenoside Rg3 Fusarium proliferatum ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 39100, SDS-PAGE Fusarium proliferatum

Synonyms

Synonyms Comment Organism
FPG
-
Fusarium proliferatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Fusarium proliferatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60
-
Fusarium proliferatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable at temperatures below 60°C. The half-lives of the purified enzyme at 30, 40, 50, 60 and 70°C are 141.4, 90.0, 58.7, 27.1 and 1.4 h, respectively Fusarium proliferatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Fusarium proliferatum

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
Fusarium proliferatum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6.5 the enzyme activity remains more than 70% after incubating at pH 4.0-6.5 at 4°C for 24 h Fusarium proliferatum

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium proliferatum isoelectric focusing
-
4.4