Any feedback?
Literature summary for 3.2.1.21 extracted from
- Construction and characterization of novel chimeric beta-glucosidases with Cellvibrio gilvus (CG) and Thermotoga maritima (TM) by overlapping PCR (2009), Biotechnol. Bioprocess Eng., 14, 266-273.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
| expressed in Escherichia coli BL21(DE3) cells | Cellulomonas gilvus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0039 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima |  |
| 0.0082 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 0.0082 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 0.44 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE | Thermotoga maritima |
| 80000 | - |
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE | Cellulomonas gilvus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas gilvus | - |
- |
- |
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and Mono-S column chromatography | Thermotoga maritima |
| Ni-NTA resin column chromatography and Mono-S column chromatography | Cellulomonas gilvus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | - |
chimeric beta-glucosidase No.8, crude extract, at 30°C | Thermotoga maritima |
| 0.14 | - |
chimeric beta-glucosidase No.8, crude extract, at 30°C | Cellulomonas gilvus |
| 0.17 | - |
chimeric beta-glucosidase No.6, crude extract, at 30°C | Thermotoga maritima |
| 0.17 | - |
chimeric beta-glucosidase No.6, crude extract, at 30°C | Cellulomonas gilvus |
| 4.53 | - |
chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C | Thermotoga maritima |
| 4.53 | - |
chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C | Cellulomonas gilvus |
| 5.55 | - |
chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C | Thermotoga maritima |
| 5.55 | - |
chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C | Cellulomonas gilvus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + D-glucopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bgl | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
optimum temperature of the wild type enzyme | Cellulomonas gilvus |
| 60 | - |
optimum temperature of No.6 and No.8 chimeric beta-glucosidases | Thermotoga maritima |
| 60 | - |
optimum temperature of No.6 and No.8 chimeric beta-glucosidases | Cellulomonas gilvus |
| 70 | - |
optimum temperature of the wild type enzyme | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | 70 | chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C above which activity decreases, the heat stability of the wild type enzyme is at 41°C | Cellulomonas gilvus |
| 60 | 87 | chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C, above which activity decreases, the heat stability of the wild type enzyme is at 41°C | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.84 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 3.84 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 5.62 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 5.62 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 6.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 42.2 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used | Thermotoga maritima |
| 3 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used | Cellulomonas gilvus |
| 4.5 | - |
the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 | Thermotoga maritima |
| 4.5 | - |
the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 | Cellulomonas gilvus |
| 5 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used | Thermotoga maritima |
| 5 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used | Cellulomonas gilvus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 95.9 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
| 468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
| 1640 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
