Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
expressed in Escherichia coli BL21(DE3) cells | Cellulomonas gilvus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
0.0082 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
0.0082 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
0.44 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE | Thermotoga maritima |
80000 | - |
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE | Cellulomonas gilvus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas gilvus | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Mono-S column chromatography | Thermotoga maritima |
Ni-NTA resin column chromatography and Mono-S column chromatography | Cellulomonas gilvus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
chimeric beta-glucosidase No.8, crude extract, at 30°C | Thermotoga maritima |
0.14 | - |
chimeric beta-glucosidase No.8, crude extract, at 30°C | Cellulomonas gilvus |
0.17 | - |
chimeric beta-glucosidase No.6, crude extract, at 30°C | Thermotoga maritima |
0.17 | - |
chimeric beta-glucosidase No.6, crude extract, at 30°C | Cellulomonas gilvus |
4.53 | - |
chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C | Thermotoga maritima |
4.53 | - |
chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C | Cellulomonas gilvus |
5.55 | - |
chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C | Thermotoga maritima |
5.55 | - |
chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C | Cellulomonas gilvus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bgl | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimum temperature of the wild type enzyme | Cellulomonas gilvus |
60 | - |
optimum temperature of No.6 and No.8 chimeric beta-glucosidases | Thermotoga maritima |
60 | - |
optimum temperature of No.6 and No.8 chimeric beta-glucosidases | Cellulomonas gilvus |
70 | - |
optimum temperature of the wild type enzyme | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | 70 | chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C above which activity decreases, the heat stability of the wild type enzyme is at 41°C | Cellulomonas gilvus |
60 | 87 | chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C, above which activity decreases, the heat stability of the wild type enzyme is at 41°C | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.84 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
3.84 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
5.62 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
5.62 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
6.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
42.2 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used | Thermotoga maritima |
3 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used | Cellulomonas gilvus |
4.5 | - |
the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 | Thermotoga maritima |
4.5 | - |
the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 | Cellulomonas gilvus |
5 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used | Thermotoga maritima |
5 | - |
the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used | Cellulomonas gilvus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95.9 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima | |
468 | - |
4-nitrophenyl-beta-D-glucopyranoside | chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C | Cellulomonas gilvus | |
1640 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C | Thermotoga maritima |